Wang Chan, Qi Longbin, Liang Rongning
Yantai Center of Shandong Integrated Technology Transfer Center, Chinese Academy of Sciences, Yantai, Shandong 264003, P. R. China.
Anal Methods. 2021 Feb 7;13(5):620-625. doi: 10.1039/d0ay02100h. Epub 2021 Jan 22.
Polymeric membrane potentiometric sensors based on molecularly imprinted polymers (MIPs) have been successfully designed for the detection of organic compounds both in ionic and neutral forms. However, most of these sensors are based on the non-covalent recognition interactions between the functional groups of the MIP in the polymeric sensing membrane and the target. These weak non-covalent interactions are unfavorable for the detection of hydrophilic organic compounds (e.g., dopamine). Herein novel MIP potentiometric sensor based covalent recognition for the determination of protonated dopamine is described. Uniform-sized boronate-based MIP beads are utilized as the recognition receptors. These receptors can covalently bind with dopamine with a cis-diol group to form a five-membered cyclic ester and thus provide a higher affinity because of the stronger nature of the covalent bonds. It has been found that the proposed electrode shows an excellent sensitivity towards dopamine with a detection limit of 2.1 μM, which could satisfy the needs for in vivo analysis of dopamine in the brain of living animals. We believe that the covalent recognition MIP-based sensing strategy provides an appealing way to design MIP-based electrochemical and optical sensors with excellent sensing properties.
基于分子印迹聚合物（MIP）的聚合物膜电位传感器已成功设计用于检测离子形式和中性形式的有机化合物。然而，这些传感器大多基于聚合物传感膜中MIP的官能团与目标物之间的非共价识别相互作用。这些弱的非共价相互作用不利于亲水性有机化合物（如多巴胺）的检测。本文描述了一种基于共价识别的新型MIP电位传感器，用于测定质子化多巴胺。尺寸均匀的硼酸基MIP珠用作识别受体。这些受体可以与具有顺式二醇基团的多巴胺共价结合形成五元环酯，因此由于共价键更强的性质而具有更高的亲和力。已发现所提出的电极对多巴胺表现出优异的灵敏度，检测限为2.1 μM，这可以满足对活体动物大脑中多巴胺进行体内分析的需求。我们相信，基于共价识别的MIP传感策略为设计具有优异传感性能的基于MIP的电化学和光学传感器提供了一种有吸引力的方法。
