Nakhleh R, Vogt J M, Edson J R
Department of Laboratory Medicine and Pathology, School of Medicine, University of Minnesota, Minneapolis.
Am J Clin Pathol. 1988 Mar;89(3):353-8. doi: 10.1093/ajcp/89.3.353.
Functional assays for heparin cofactor II (HC-II) are based on the inactivation of thrombin by HC-II in the presence of dermatan sulfate (DS). Residual thrombin is measured in a chromogenic assay. Interference by the antithrombin-III (AT-III)/heparin complex, which also rapidly inactivates thrombin, must be eliminated from the HC-II test system. Commercial DS is contaminated with heparin, while plasma specimens to be tested contain AT-III. After NaNO2/acetic acid treatment of DS (to inactivate heparin), there was enough residual heparin to cause AT-III interference. Treatment of plasma with commercially available anti-AT-III antiserum largely, but not completely, removed AT-III interference from the HC-II assay. With commercially available reagents, both NaNO2/acetic acid treatment of DS and anti-AT-III treatment of plasma were needed to eliminate heparin/AT-III interference. Protamine sulfate inactivated DS as well as heparin and could not be used to reduce AT-III/heparin interference with the HC-II assay.
肝素辅因子II（HC-II）的功能测定基于在硫酸皮肤素（DS）存在下，HC-II使凝血酶失活。在显色测定中测量残余凝血酶。抗凝血酶III（AT-III）/肝素复合物也能迅速使凝血酶失活，其干扰必须从HC-II测试系统中消除。市售的DS被肝素污染，而待测血浆标本中含有AT-III。用NaNO2/乙酸处理DS（使肝素失活）后，仍有足够的残余肝素引起AT-III干扰。用市售抗AT-III抗血清处理血浆在很大程度上，但不是完全消除了HC-II测定中的AT-III干扰。使用市售试剂时，需要对DS进行NaNO2/乙酸处理以及对血浆进行抗AT-III处理，以消除肝素/AT-III干扰。硫酸鱼精蛋白可使DS以及肝素失活，不能用于减少AT-III/肝素对HC-II测定的干扰。
