NMI Natural and Medical Sciences, Institute at the University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, Germany and Department of Women's Health, Research Institute for Women's Health, Eberhard Karls University Tübingen, Silcherstr. 7/1, 72076 Tübingen, Germany.
Institute of Physical and Theoretical Chemistry, Eberhard Karls University Tübingen, Auf der Morgenstelle 18, 72076 Tübingen, Germany.
J Mater Chem B. 2021 Feb 14;9(6):1647-1660. doi: 10.1039/d0tb02757j. Epub 2021 Jan 22.
Fibronectin coating increases implant biocompatibility by enhancing surface endothelialization via integrin-mediated binding. Surface properties determine the fibronectin orientation and conformation, dictating which ligands are presented, and therefore altering the bioactivity of an implant surface. In this study, polyurethane was treated with oxygen plasma, which allowed for a simultaneous modification of the surface chemistry and topography to modulate fibronectin adsorption. By varying the parameters of the treatment, human plasma fibronectin adsorbed on the surfaces in different conformations, orientations, and binding affinities, which was investigated by atomic force microscopy, fluorescence microscopy, monoclonal and polyclonal antibody staining and reflectometric interference spectroscopy. Apart from the most hydrophilic rough surfaces, the adsorbed fibronectin showed a lower binding affinity and less conformational change on the more hydrophilic surfaces. A large amount of exposed fibronectin-cell binding was detected on the rough treated and the smooth untreated surfaces. Primary isolated human umbilical vein and human microvascular endothelial cells showed a significantly higher cell adherence on the absorbed fibronectin with a low binding affinity and low conformational changes. Significant differences in the formation of mature focal adhesions and the reorganization of F-actin were identified on the rough treated and the smooth untreated surfaces. Our data suggest that oxygen plasma treatment is a reliable technique for the modulation of fibronectin adsorption in order to adjust fibronectin bioactivity and impact cell responses to implant surfaces.
纤维连接蛋白涂层通过整合素介导的结合增强表面内皮化来提高植入物的生物相容性。表面特性决定了纤维连接蛋白的取向和构象，决定了哪些配体被呈现，从而改变植入物表面的生物活性。在这项研究中，通过氧等离子体处理了聚氨酯，从而可以同时修饰表面化学和形貌，以调节纤维连接蛋白的吸附。通过改变处理参数，人血浆纤维连接蛋白以不同的构象、取向和结合亲和力吸附在表面上，这通过原子力显微镜、荧光显微镜、单克隆和多克隆抗体染色和反射干涉光谱进行了研究。除了最亲水的粗糙表面外，在更亲水的表面上，吸附的纤维连接蛋白表现出较低的结合亲和力和较小的构象变化。在粗糙处理的表面和未处理的光滑表面上检测到大量暴露的纤维连接蛋白-细胞结合。从人脐静脉和人微血管内皮细胞中分离出的原代细胞在具有低结合亲和力和低构象变化的吸附纤维连接蛋白上表现出明显更高的细胞黏附。在粗糙处理的表面和未处理的光滑表面上，成熟的粘着斑的形成和 F-肌动蛋白的重排存在显著差异。我们的数据表明，氧等离子体处理是一种可靠的技术，可以调节纤维连接蛋白的吸附，以调整纤维连接蛋白的生物活性并影响细胞对植入物表面的反应。
