Enhanced denitrification performance of strain YSF15 by different molecular weight of humic acid: Mechanism based on the biological products and activity.

This study aimed to clarify the facilitation by humic acid (HA) fractions (>100, 100-50, 50-30, 30-10, 10-3 and < 3 kDa), as well as their variable effect on denitrification of strain YSF15 under low carbon-nitrogen ratio and nitrate conditions. All HA fractions with 7 mg L were able to accelerate nitrate removal by strain YSF15 and the role of carbon source was inconspicuous. The molecular weight (MW) < 3 kDa was the best promoter for denitrification, with the efficiency (91.32%) far exceeding the control (43.27%), resulting in more stable oxidation-reduction potential, higher nutrients utilization and electron transport activity, more compact protein structure in extracellular polymeric substances and the production of endogenous HA. Each HA fraction could change the bio-products and denitrification activity of strain YSF15. This study sheds light on the facilitation of HA in denitrification from the perspective of MW, implying the potential effect of HA on denitrifying bacteria in community.