Genome Biotechnology Laboratory, Kanazawa Institute of Technology, 3-1 Yatsukaho, Hakusan, Ishikawa 924-0838, Japan.
Genome Biotechnology Laboratory, Kanazawa Institute of Technology, 3-1 Yatsukaho, Hakusan, Ishikawa 924-0838, Japan; Research Laboratory for Integrated Technological Systems, Kanazawa Institute of Technology, 3-1 Yatsukaho, Hakusan, Ishikawa 924-0838, Japan.
J Biosci Bioeng. 2021 Apr;131(4):390-395. doi: 10.1016/j.jbiosc.2020.12.007. Epub 2021 Jan 20.
Fungal sporulation is affected by many environmental factors, for example, we previously observed that embedding of a hydrophobic polymer net in an agar plate medium significantly accelerates spore formation of some fungi. Here, it was found that the fungal spore formation depended on the surface hydrophobicity of cultivation vessels used for the plate cultivation. In a polypropylene (PP) vessel, six fungal strains produced spores of 1.5 to 514.8 times of those growing in a glass vessel. The contact of vegetative hyphae on the surface of the vessels might trigger the fungal spore formation. Moreover, the spore formation was synergistically accelerated by the reduction of nitrogen source content in an agar plate medium and by the contact to hydrophobic polymers. The synergistic effect depended on the surface area of the hydrophobic polymer. Thus, the combination of the reduction of nitrogen source and the embedding of hydrophobic polymer is expected as a novel and effective procedure for production of fungal spores which are useful for the inoculum in fermentation industry and biocontrol agent in agriculture.
真菌的孢子形成受到许多环境因素的影响，例如，我们之前观察到，在琼脂平板培养基中嵌入疏水聚合物网会显著加速某些真菌的孢子形成。在这里，我们发现真菌的孢子形成取决于用于平板培养的培养容器的表面疏水性。在聚丙烯 (PP) 容器中，六种真菌菌株产生的孢子是在玻璃容器中生长的孢子的 1.5 到 514.8 倍。营养菌丝在容器表面的接触可能会触发真菌的孢子形成。此外，氮源含量的降低和与疏水聚合物的接触协同加速了孢子的形成。协同效应取决于疏水聚合物的表面积。因此，减少氮源和嵌入疏水聚合物的组合有望成为一种生产真菌孢子的新方法，这种方法对于发酵工业中的接种物和农业中的生物防治剂非常有用。
