Department of Hepatobiliary Surgery, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China.
Clinic Medicine Research Center of Hepatobiliary Diseases, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China.
J Cell Mol Med. 2021 Apr;25(8):3793-3802. doi: 10.1111/jcmm.16254. Epub 2021 Jan 23.
Hepatocellular carcinoma (HCC) is one of the most common cancers with high prevalence and mortality, and it has brought huge economic and health burden for the world. It is urgent to found novel targets for HCC diagnosis and clinical intervention. Circular RNA (circRNA) has been reported to participate in many cancer progressions including HCC, suggesting that circRNA might paly essential role in HCC initiation and progression. Our study aims to found that potential circRNA participates in HCC development and its underlying molecular mechanisms. We obtained three pairs of HCC tissues and its adjacent normal tissues data from GEO DataSets. MTT, cell colony, EdU, wound-healing, transwell invasion and mouse xenograft model assays were used to demonstrate the biological functions of circCAMSAP1 in HCC progression. Furthermore, we conducted bioinformatics analysis, AGO2-RIP, RNA pull-down and luciferase reporter assays to assess the association of circCAMSAP1-miR-1294-GRAMD1A axis in HCC cells. The expression of circCAMSAP1 was up-regulated in HCC tissues compared with its adjacent normal tissues. Up-regulation of circCAMSAP1 promoted HCC biological functions both in vitro and in vivo. The promotive effects of circCAMSAP1 on HCC progression function through miR-1294/GRAMD1A pathway. CircCAMSAP1 was up-regulated in HCC tissues, and circCAMSAP1 up-regulated GRAMD1A expression to promote HCC proliferation, migration and invasion through miR-1294. CircCAMSAP1 might be a potential prognosis and therapeutic target for HCC.
肝细胞癌 (HCC) 是最常见的癌症之一，具有较高的发病率和死亡率，给世界带来了巨大的经济和健康负担。因此，迫切需要发现 HCC 诊断和临床干预的新靶点。环状 RNA (circRNA) 已被报道参与多种癌症的进展，包括 HCC，表明 circRNA 可能在 HCC 的发生和发展中发挥重要作用。我们的研究旨在发现潜在的 circRNA 参与 HCC 的发展及其潜在的分子机制。我们从 GEO DataSets 中获得了三对 HCC 组织及其相邻正常组织的数据。MTT、细胞集落形成、EdU、划痕愈合、transwell 侵袭和小鼠异种移植模型实验用于证明 circCAMSAP1 在 HCC 进展中的生物学功能。此外，我们进行了生物信息学分析、AGO2-RIP、RNA 下拉和荧光素酶报告基因检测，以评估 circCAMSAP1-miR-1294-GRAMD1A 轴在 HCC 细胞中的关联。与相邻正常组织相比，circCAMSAP1 在 HCC 组织中表达上调。circCAMSAP1 的上调促进了 HCC 在体外和体内的生物学功能。circCAMSAP1 通过 miR-1294/GRAMD1A 通路促进 HCC 进展功能。CircCAMSAP1 在 HCC 组织中上调，circCAMSAP1 通过 miR-1294 上调 GRAMD1A 的表达，促进 HCC 的增殖、迁移和侵袭。CircCAMSAP1 可能是 HCC 的一个有潜力的预后和治疗靶点。
