State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046, China; Joint International Research Centre for Critical Zone Science-University of Leeds and Nanjing University, Nanjing University, Nanjing 210023, China.
School of Environment, Nanjing Normal University, Nanjing 210036, China.
Sci Total Environ. 2021 May 15;769:145224. doi: 10.1016/j.scitotenv.2021.145224. Epub 2021 Jan 16.
Plant-associated microorganisms play an important role in controlling heavy metal uptake and accumulation in aerial parts. The microbial community and its interaction with Cd accumulation by willow were assessed to explore the association of phytoextraction efficiency and rhizospheric microbial populations. Therefore, the rhizosphere microbial compositions of three willow genotypes grown in two Cd polluted sites were investigated, focusing on their interactions with phytoremediation potential. Principal coordinate analysis revealed a significant effect of genotype on the rhizosphere microbial communities. Distinct beneficial microorganisms, such as plant growth promoting bacteria (PGPB) and mycorrhizal fungi, were assembled in the rhizosphere of different willow genotypes. Linear mixed models showed that the relative abundance of PGPB was positively associated (p < 0.01) with Cd accumulation, since these microbes significantly increased willow growth. The higher abundance of arbuscular mycorrhizal fungi in the rhizosphere of Salix × aureo-pendula CL 'J1011' at the Kejing site, showed a negative correlation with the Cd content, but a positive correlation with biomass. Conversely, mycorrhizal fungi, were more abundant in the rhizosphere of S. × jiangsuensis CL. 'J2345' and positively correlated with the Cd content in willow tissues. This study provides new insights into the distinctive microbial communities in rhizosphere of different willow genotypes, which may be consistent with the phytoremediation potential.
植物相关微生物在控制地上部分重金属吸收和积累方面发挥着重要作用。评估微生物群落及其与柳树 Cd 积累的相互作用，以探索植物提取效率和根际微生物种群之间的关系。因此，研究了在两个 Cd 污染点生长的三种柳树基因型的根际微生物组成，重点研究它们与植物修复潜力的相互作用。主坐标分析显示基因型对根际微生物群落有显著影响。不同柳树基因型的根际中聚集了明显有益的微生物，如植物促生菌（PGPB）和菌根真菌。线性混合模型显示，PGPB 的相对丰度与 Cd 积累呈正相关（p<0.01），因为这些微生物显著促进了柳树的生长。在科景点的金叶柳（Salix × aureo-pendula CL 'J1011'）根际中，丛枝菌根真菌的丰度较高，与 Cd 含量呈负相关，但与生物量呈正相关。相反，在江苏柳（S. × jiangsuensis CL. 'J2345'）的根际中，菌根真菌更为丰富，与柳树组织中的 Cd 含量呈正相关。本研究为不同柳树基因型根际中独特的微生物群落提供了新的见解，这可能与植物修复潜力一致。
