Walker R L, LeaMaster B R, Biberstein E L, Stellflug J N
Department of Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis 95616.
Am J Vet Res. 1988 Feb;49(2):208-12.
An ELISA for the detection of antibodies to Histophilus ovis was used to evaluate the association of epididymal lesions in rams with serologic response to His ovis. Comparison of ELISA results for His ovis in groups of rams with epididymal lesions with ELISA results of clinically normal rams (control group) revealed a significant difference (P less than 0.01) between the control group and those rams from which His ovis was isolated. A significant difference (P less than 0.01) was noticed between the control group and rams with lesions from which an organism other than His ovis or Brucella ovis was isolated. Additionally, a significant difference (P less than 0.01) was noticed in ELISA results between the control group and affected rams from which no organism was recovered and in which the epididymal lesion was not limited to the head of the epididymis. A difference was not detected in the His ovis ELISA results between control rams and rams with lesions associated with a B ovis infection or rams from which no organism was recovered and in which the epididymal lesion was limited to the head of the epididymis. The serologic findings in our study suggest that His ovis is more important in the development of epididymitis in rams than culture results alone would indicate.
采用一种用于检测绵羊嗜组织菌抗体的酶联免疫吸附测定（ELISA）来评估公羊附睾病变与对绵羊嗜组织菌血清学反应之间的关联。将患有附睾病变的公羊组中绵羊嗜组织菌的ELISA结果与临床正常公羊（对照组）的ELISA结果进行比较，发现对照组与分离出绵羊嗜组织菌的公羊之间存在显著差异（P<0.01）。在对照组与分离出除绵羊嗜组织菌或绵羊布鲁氏菌以外的其他微生物的有病变公羊之间也注意到显著差异（P<0.01）。此外，在对照组与未分离出微生物且附睾病变不限于附睾头部的受影响公羊的ELISA结果之间也注意到显著差异（P<0.01）。在对照组公羊与伴有绵羊布鲁氏菌感染相关病变的公羊之间，或在未分离出微生物且附睾病变仅限于附睾头部的公羊之间，未检测到绵羊嗜组织菌ELISA结果存在差异。我们研究中的血清学结果表明，绵羊嗜组织菌在公羊附睾炎的发生发展中比单纯培养结果所显示的更为重要。
