Institute of Environmental Technology, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.
Institute of Environmental Technology, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.
Environ Int. 2021 Mar;148:106394. doi: 10.1016/j.envint.2021.106394. Epub 2021 Jan 22.
Existing drinking water treatment systems have limited ability to control emerging contaminants such as antibiotic resistance genes (ARGs). Household activated carbon water purifiers (HWPs) are convenient measures to assure drinking water quality. However, ARGs distribution in HWPs has not been reported. Here, ARGs, mobile genetic elements (MGEs) and bacteria communities were profiled in tap water (TW), filter water (FW) and activated carbon (AC) biofilm from six kinds of HWPs after 80 days operation, using metagenomics. Results showed that the bacteria community diversities in FW and AC were higher than those in TW. A total of 88, 116 and 80 ARG subtypes were detected in TW, AC and FW, respectively. The AC structure was an important factor influencing the bacterial communities and ARG profiles in FW. The network analysis revealed the co-occurrence patterns between ARGs and bacteria. SourceTracker analyses showed AC biofilms were important contributors of microbes (29-79%) and ARGs (17-53%) in FW. Moreover, MGEs e.g. pBBta01, pMKMS02 and pMFLV01 plasmids, and ISMysp3 had significant co-occurrence patterns with ARGs in the AC biofilms. This study helps to understand the actual purification effect of HWPs and provides a theoretical reference for the management and control of ARGs pollution in domestic drinking water.
现有的饮用水处理系统对新兴污染物（如抗生素耐药基因 [ARGs]）的控制能力有限。家用活性炭水净化器（HWPs）是保证饮用水质量的便捷措施。然而，HWPs 中 ARGs 的分布尚未报道。在这里，使用宏基因组学方法，在经过 80 天运行后，对来自六种 HWPs 的自来水（TW）、过滤水（FW）和活性炭（AC）生物膜中的 ARGs、移动遗传元件（MGEs）和细菌群落进行了分析。结果表明，FW 和 AC 中的细菌群落多样性高于 TW。在 TW、AC 和 FW 中分别检测到 88、116 和 80 种 ARG 亚型。AC 结构是影响 FW 中细菌群落和 ARG 谱的重要因素。网络分析揭示了 ARGs 与细菌之间的共现模式。SourceTracker 分析表明，AC 生物膜是 FW 中微生物（29-79%）和 ARGs（17-53%）的重要来源。此外，MGEs 如 pBBta01、pMKMS02 和 pMFLV01 质粒和 ISMysp3 与 AC 生物膜中的 ARGs 存在显著的共现模式。本研究有助于了解 HWPs 的实际净化效果，并为家用饮用水中 ARGs 污染的管理和控制提供理论参考。
