Irimajiri N, Haneda J, Yokoyama E, Shirakabe M, Matsumoto M, Kusunoki T, Utsunomiya J
2nd Dept. of Surgery, Hyogo Medical College, Japan.
Gan To Kagaku Ryoho. 1988 Mar;15(3):449-55.
Six-day SRC assay as a chemosensitivity test has an advantage of high predictive rate for clinical response. However, it is pointed out that very few viable tumor cells are observed at the end of the assay, so that it may make the assay results unreliable. In this paper, we tested the effect of immunosuppressants on SRC assay using Walker carcinosarcoma originated from Wistar rat xenografted under the renal capsule of BDF1 mice. The changes of tumor size, pathological features and proliferative ability of xenografted tumor under the renal capsule of mice treated with cyclophosphamide, mizolibine or cyclosporin A are examined. Only cyclosporin A treatment could maintain the viable tumor cells and proliferative ability of the tumor grafted under the renal capsule 21 days after transplantation. In order to compare the original 6-day SRC assay developed by Bogden et al, we applied immunosuppressants to the 6-day assay. It is suggested that cyclosporin A and mizolibine amplify the sensitivity of tumor in 6-day SRC assay.
作为一种化学敏感性测试，为期六天的软琼脂集落形成试验（SRC试验）对临床反应具有较高的预测率优势。然而，有人指出，在试验结束时观察到的存活肿瘤细胞极少，这可能会使试验结果不可靠。在本文中，我们使用源自Wistar大鼠的Walker癌肉瘤，将其移植到BDF1小鼠肾被膜下，测试了免疫抑制剂对SRC试验的影响。研究了用环磷酰胺、咪唑立宾或环孢素A处理的小鼠肾被膜下移植瘤的肿瘤大小、病理特征和增殖能力的变化。只有环孢素A处理能够在移植后21天维持肾被膜下移植瘤的存活肿瘤细胞和增殖能力。为了比较Bogden等人最初开发的为期六天的SRC试验，我们在为期六天的试验中应用了免疫抑制剂。结果表明，在为期六天的SRC试验中，环孢素A和咪唑立宾可增强肿瘤的敏感性。
