[采用BDF1小鼠肾包膜下移植法对人乳腺癌异种移植瘤（MX-1）进行化学敏感性分析]

[Analysis of chemosensitivity against a human breast cancer xenograft (MX-1) with the subrenal capsule assay in BDF1 mice].

作者信息

Inoue K, Wang Y, Tada A, Ueno K, Kojima K, Ogawa M

出版信息

Gan To Kagaku Ryoho. 1985 Aug;12(8):1644-50.

Abstract

The antitumor activity of 27 anticancer drugs against a human breast cancer tumor (MX-1) has been studied using the subrenal capsule assay developed by A.E. Bogden et al. in 1978. Without immunosuppression with cyclophosphamide, BDF1 mice transplanted with MX-1 were treated with various drugs. The antitumor activity was evaluated by the tumor growth inhibition rate on day 6 after treatment. Among the 27 anticancer drugs tested, 10 compounds (37%) which showed more than 75% tumor growth inhibition rate were considered to be active. On the other hand, 8 compounds out of 27 drugs (30%) which showed less than 50% tumor growth inhibition rate were considered to be inactive. When the antitumor activity between the subrenal capsule assay in BDF1 mice and the subcutaneous transplantation assay in nude mice were compared, both assays were well correlated (r = 0.787, p less than 0.001). These results suggest that the antitumor activity of drugs can be evaluated faster, cheaper and easier by the subrenal capsule assay compared with the subcutaneous transplantation assay in nude mice.

摘要

利用A.E. 博登等人于1978年开发的肾包膜下移植法，研究了27种抗癌药物对人乳腺癌肿瘤（MX-1）的抗肿瘤活性。在不使用环磷酰胺进行免疫抑制的情况下，对移植了MX-1的BDF1小鼠使用各种药物进行治疗。通过治疗后第6天的肿瘤生长抑制率来评估抗肿瘤活性。在所测试的27种抗癌药物中，有10种化合物（37%）的肿瘤生长抑制率超过75%，被认为具有活性。另一方面，27种药物中有8种化合物（30%）的肿瘤生长抑制率低于50%，被认为无活性。当比较BDF1小鼠肾包膜下移植法和裸鼠皮下移植法的抗肿瘤活性时，两种方法相关性良好（r = 0.787，p < 0.001）。这些结果表明，与裸鼠皮下移植法相比，肾包膜下移植法能够更快、更便宜且更简便地评估药物的抗肿瘤活性。