Terashima M, Ikeda K, Kawamura S, Satoh M, Ishida K, Amano K, Takagane A, Yaegashi Y, Saitoh K
1st Dept. of Surgery, Iwate Medical University.
Gan To Kagaku Ryoho. 1988 Mar;15(3):505-11.
For the purpose to suppress the host immune reaction in subrenal capsule assay (SRCA) using normal immunocompetent mice, the effects of cyclophosphamide (CPM) pre-treatment (CPM 180 mg/kg day-1 s.c.) were compared with cyclosporine A (CSA) treatment (CSA 60 mg/kg s.c. daily). CPM and CSA suppressed host reaction until day 6 and day 12, respectively. However, in the histological evaluation of day 6 tumor, there was no difference between two groups. Mouse serum levels of CPM were measured by a bioassay as indicated by the cytotoxicity against P388 cells, to evaluate its residual activity against implanted tumor. No cytotoxicity was observed in the serum of 36 hr. after CPM injection. The antitumor activities of several chemotherapeutic agents against serially transplanted human esophageal cancer xenograft (IMEs-1) in untreated control group and CPM pre-treated group of SRCA were compared with the subcutaneous transplantation assay in nude mice. In all agents, the drug activities in CPM pre-treated group were similar to that in nude mice assay system. The increase of toxicity in CPM pre-treated group was very light. The immunosuppressive activity of CPM were also tested in 14 clinical samples. CPM suppressed the host reaction satisfactorily in all samples. These results suggest the usefulness of CPM pretreatment for suppressing the host reaction in SRCA.
为了在使用正常免疫活性小鼠的肾包膜下试验（SRCA）中抑制宿主免疫反应，比较了环磷酰胺（CPM）预处理（CPM 180 mg/kg皮下注射，每日1次）与环孢素A（CSA）治疗（CSA 60 mg/kg皮下注射，每日1次）的效果。CPM和CSA分别在第6天和第12天之前抑制宿主反应。然而，在第6天肿瘤的组织学评估中，两组之间没有差异。通过生物测定法测量小鼠血清中的CPM水平，该生物测定法通过对P388细胞的细胞毒性来指示，以评估其对植入肿瘤的残留活性。在CPM注射后36小时的血清中未观察到细胞毒性。将几种化疗药物在未处理的对照组和SRCA的CPM预处理组中对连续移植的人食管癌异种移植瘤（IMEs-1）的抗肿瘤活性与裸鼠皮下移植试验进行了比较。在所有药物中，CPM预处理组的药物活性与裸鼠试验系统中的相似。CPM预处理组中毒性的增加非常轻微。还在14个临床样本中测试了CPM的免疫抑制活性。CPM在所有样本中均能令人满意地抑制宿主反应。这些结果表明CPM预处理在抑制SRCA中的宿主反应方面是有用的。
