褪黑素对雄性大鼠内脏脂肪沉积、脂代谢及肝脂代谢基因表达的影响。
Effect of melatonin on visceral fat deposition, lipid metabolism and hepatic lipo-metabolic gene expression in male rats.
机构信息
College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, China.
Department of Animal Science, Cornell University, Ithaca, NY, USA.
出版信息
J Anim Physiol Anim Nutr (Berl). 2021 Jul;105(4):787-796. doi: 10.1111/jpn.13497. Epub 2021 Jan 23.
Melatonin (MT) influences lipid metabolism in animals; however, the mechanistic effect of melatonin on liver fat and abdominal adipose deposition requires further clarity. In order to study the effects of melatonin on lipid metabolism, and hepatic fat and abdominal adipose deposition in animals, twenty Sprague-Dawley (SD) rats of 6 weeks of age with similar bodyweight were randomly divided into two groups: control (CTL) and MT-treated (10 mg/kg/day). During a 60-day experiment, food intake and bodyweight were measured daily and weekly respectively. At the end of treatment, blood samples were collected to collect plasma to quantify hormones and metabolic indicators of lipid metabolism. In addition, organ and abdominal adipose depots including liver, and omental, perirenal, and epididymal fat were weighed. Liver tissue was sampled for sectioning, long-chain fatty acid (LCFA) quantification, and gene chip and Real-time quantitative PCR (qPCR) analyses. The results showed that liver weight and index (ratio of liver weight to body weight) in MT group reduced by 20.69% and 9.63% respectively; omentum weight and index reduced by 59.88% and 54.93% respectively, and epididymal fat weight reduced by 45.34% (p = 0.049), relative to CTL. Plasma lipid indices, triglyceride (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL) and total cholesterol (TC) with MT treatment decreased significantly compared with the control. Fat and 8 LCFA content in liver in MT group also decreased. Gene chip and qPCR demonstrated that there were 289 genes up-regulated and 293 genes down-regulated by MT. Further analysis found that the mRNA expression of lipolysis-related genes increased, while the mRNA expression of lipogenesis-related enzymes decreased (p < 0.05) with MT. This study concluded that melatonin greatly affected fat deposition, and hepatic LCFA supply and the expression of genes associated with lipogenesis and lipolysis.
褪黑素 (MT) 影响动物的脂质代谢；然而，褪黑素对肝脏脂肪和腹部脂肪沉积的机制影响仍需进一步阐明。为了研究褪黑素对动物脂质代谢、肝脂肪和腹部脂肪沉积的影响，将 20 只 6 周龄、体重相近的 Sprague-Dawley（SD）大鼠随机分为两组：对照组（CTL）和褪黑素处理组（10mg/kg/天）。在 60 天的实验中，分别每天和每周测量食物摄入量和体重。治疗结束时，采集血样以收集血浆，以定量测定激素和脂质代谢的代谢指标。此外，还称取包括肝脏在内的器官和腹部脂肪沉积，以及大网膜、肾周和附睾脂肪。采集肝组织进行切片、长链脂肪酸（LCFA）定量以及基因芯片和实时定量 PCR（qPCR）分析。结果表明，MT 组的肝重和肝重指数（肝重与体重的比值）分别降低了 20.69%和 9.63%；大网膜重和指数分别降低了 59.88%和 54.93%，附睾脂肪重降低了 45.34%（p=0.049），与 CTL 相比。与对照组相比，MT 处理后血浆脂质指标、甘油三酯（TG）、高密度脂蛋白（HDL）、低密度脂蛋白（LDL）和总胆固醇（TC）显著降低。MT 组肝脏中的脂肪和 8 种 LCFA 含量也降低。基因芯片和 qPCR 表明，MT 有 289 个基因上调和 293 个基因下调。进一步分析发现，MT 处理后与脂肪分解相关的基因的 mRNA 表达增加，而与脂肪生成相关的酶的 mRNA 表达减少（p<0.05）。本研究得出结论，褪黑素对脂肪沉积、肝脏 LCFA 供应以及与脂肪生成和脂肪分解相关的基因表达有很大影响。
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