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NIN 样蛋白 7 通过激活色氨酸转氨酶相关 2 的转录促进硝酸盐介导的侧根发育。

NIN-like protein 7 promotes nitrate-mediated lateral root development by activating transcription of TRYPTOPHAN AMINOTRANSFERASE RELATED 2.

机构信息

Xinjiang Production and Construction Corps Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization, Department of Horticulture, College of Agriculture, Shihezi University, Shihezi, 832003, Xinjiang, China; State Key Laboratory of Crop Biology, Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, 271018, Shandong, China.

State Key Laboratory of Crop Biology, Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, 271018, Shandong, China.

出版信息

Plant Sci. 2021 Feb;303:110771. doi: 10.1016/j.plantsci.2020.110771. Epub 2020 Nov 25.

Abstract

Nitrate is essential for plant growth and development. When nitrate availability is low, plants produce more lateral roots (LRs) to seek nitrate from the soil. In this study, by DNA electrophoretic mobility shift and luciferase assays, it was showed that NIN-like protein 7 (NLP7) transcription factor activated expression of TAR2 by directly binding to its promoter. Finally, through genetic analysis, it was speculated that NLP7 regulated LR development through TAR2. In conclusion, NLP7 binds to the TAR2 promoter and activates TAR2 expression, thereby promoting nitrate-dependent LR development.

摘要

硝酸盐是植物生长和发育所必需的。当硝酸盐供应不足时,植物会产生更多的侧根(LRs),从土壤中寻找硝酸盐。在这项研究中,通过 DNA 电泳迁移率变动分析和荧光素酶分析,发现 NIN 样蛋白 7(NLP7)转录因子通过直接结合其启动子激活 TAR2 的表达。最后,通过遗传分析,推测 NLP7 通过 TAR2 调节 LR 的发育。总之,NLP7 与 TAR2 启动子结合并激活 TAR2 的表达,从而促进硝酸盐依赖的 LR 发育。

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