Eglitis M A, Kohn D B, Moen R C, Blaese R M, Anderson W F
Laboratory of Molecular Hematology, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892.
Biochem Biophys Res Commun. 1988 Feb 29;151(1):201-6. doi: 10.1016/0006-291x(88)90579-7.
In an effort to determine if viral envelope type influences the infectivity of human hematopoietic progenitor cells with retroviral vectors, we have pseudotyped the retroviral vector N2, which confers G418-resistance, in either an amphotropic or xenotropic envelope. Vector titres obtained by the pseudotype procedure were nearly two orders of magnitude lower than the titer obtained when N2 was packaged using the amphotropic PA317 packaging cell line. Despite its low titer, xenotropically pseudotyped N2 generated G418-resistant hematopoietic colonies at levels approaching those observed after bone marrow was infected using vector packaged using PA317 cells. These results suggest that manipulations of vector envelope may lead to improvements in the level of infection of human hematopoietic stem cells.
为了确定病毒包膜类型是否会影响逆转录病毒载体对人类造血祖细胞的感染性，我们用嗜异性或亲异性包膜对赋予G418抗性的逆转录病毒载体N2进行了假型化。通过假型化程序获得的载体滴度比使用嗜异性PA317包装细胞系包装N2时获得的滴度低近两个数量级。尽管其滴度较低，但亲异性假型化的N2产生的G418抗性造血集落水平接近使用PA317细胞包装的载体感染骨髓后观察到的水平。这些结果表明，对载体包膜的操作可能会提高人类造血干细胞的感染水平。
