Gressner A M, Haarmann R
Department of Clinical Chemistry, Phillipps-University, Marburg, FRG.
Biochem Biophys Res Commun. 1988 Feb 29;151(1):222-9. doi: 10.1016/0006-291x(88)90582-7.
The ability of rat liver fat storing cells to synthesize and to secrete hyaluronic acid was examined in monolayer cultures. The cells produce [3H] glucosamine-labeled hyaluronic acid, of which about 80% are secreted into the medium. The synthesis rate per cell (mg DNA) of labeled total glycosaminoglycans and hyaluronic acid in the medium increases significantly with culture time, but hyaluronic acid expressed as fraction of total glycosaminoglycans declines from about 0.70 in early cultures (up to the 4th day) down to 0.20 in advanced cultures. Cycloheximide increases and beta-D-xylopyranoside decreases significantly the fraction of hyaluronic acid in the medium, colchicine up to 5 microM was without effect. The synthesis of hyaluronic acid is a newly recognized function of this special type of sinusoidal liver cells. The results suggest that fat storing cells are likely to be a major source of hyaluronic acid in normal and probably also in injured liver.
在单层培养中检测了大鼠肝脏贮脂细胞合成和分泌透明质酸的能力。这些细胞产生[3H]葡糖胺标记的透明质酸，其中约80%分泌到培养基中。培养基中标记的总糖胺聚糖和透明质酸的每细胞合成速率（mg DNA）随培养时间显著增加，但以总糖胺聚糖的比例表示的透明质酸从早期培养（直至第4天）的约0.70下降到晚期培养的0.20。放线菌酮显著增加而β-D-吡喃木糖苷显著降低培养基中透明质酸的比例，高达5 microM的秋水仙碱无作用。透明质酸的合成是这种特殊类型的肝血窦细胞新发现的功能。结果表明，贮脂细胞可能是正常肝脏以及可能损伤肝脏中透明质酸的主要来源。
