Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Proteomics Research Center, School of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Iran J Med Sci. 2021 Jan;46(1):43-51. doi: 10.30476/ijms.2019.82120.0.
Cutaneous leishmaniasis caused by species () is one of the most important parasitic diseases in humans. To gain information on the metabolite variations and biochemical pathways between , we used the comparative metabolome of metacyclic promastigotes in the Iranian isolates of and by proton nuclear magnetic resonance (H-NMR).
and were collected from three areas of Iran, namely Gonbad, Mashhad, and Bam, between 2017 and 2018, and were cultured. The metacyclic promastigote of each species was separated, and cell metabolites were extracted. H-NMR spectroscopy was applied, and the data were processed using ProMatab in MATLAB (version 7.8.0.347). Multivariate statistical analyses, including the principal component analysis and the orthogonal projections to latent structures discriminant analysis, were performed to identify the discriminative metabolites between the two . Metabolites with variable influences in projection values of more than one and a P value of less than 0.05 were marked as significant differences.
A set of metabolites were detected, and 24 significantly differentially expressed metabolites were found between the metacyclic forms of and isolates. The top differential metabolites were methionine, aspartate, betaine, and acetylcarnitine, which were increased more in than (P<0.005), whereas asparagine, 3-hydroxybutyrate, L-proline, and kynurenine were increased significantly in (P<0.01). The significantly altered metabolites were involved in eight metabolic pathways.
Metabolomics, as an invaluable technique, yielded significant metabolites, and their biochemical pathways related to the metacyclic promastigotes of and . The findings offer greater insights into parasite biology and how pathogens adapt to their hosts.
由 种（）引起的皮肤利什曼病是人类最重要的寄生虫病之一。为了获得 和 伊朗分离株循环期前体鞭毛体之间代谢物变化和生化途径的信息，我们采用质子核磁共振（H-NMR）对其进行了比较代谢组学研究。
2017 年至 2018 年，我们从伊朗的 Gonbad、Mashhad 和 Bam 三个地区收集 和 并进行培养。分离出各物种的循环期前体鞭毛体，提取细胞代谢物。采用 H-NMR 光谱法，使用 MATLAB（版本 7.8.0.347）中的 ProMatab 对数据进行处理。采用主成分分析和正交偏最小二乘判别分析等多变量统计分析方法，识别两种 之间的鉴别代谢物。投影变量值大于 1 且 P 值小于 0.05 的代谢物被标记为有显著差异。
检测到一组代谢物，发现 和 分离株循环期前体鞭毛体之间有 24 种差异表达的显著差异代谢物。差异最大的代谢物为蛋氨酸、天冬氨酸、甜菜碱和乙酰肉碱，它们在 中比 （P<0.005）增加更多，而天冬酰胺、3-羟基丁酸、L-脯氨酸和犬尿氨酸在 中显著增加（P<0.01）。显著改变的代谢物涉及 8 条代谢途径。
代谢组学作为一种非常有价值的技术，产生了与 和 循环期前体鞭毛体相关的重要代谢物及其生化途径。这些发现为寄生虫生物学和病原体如何适应宿主提供了更深入的了解。
