Ueda K, Isohashi F, Okamoto K, Kokuhu I, Kimura K, Yoshikawa K, Sakamoto Y
Department of Dermatology, Osaka University Medical School, Japan.
Biochem Biophys Res Commun. 1988 Mar 15;151(2):763-7. doi: 10.1016/s0006-291x(88)80346-2.
"Activated" glucocorticoid-receptor complexes purified about 3,000-fold from rat liver were found to bind to histone-agarose. Because of their tight binding, they could not be eluted from the column by high salt solution (3 M KCl) or low salt plus polyol buffer (50% ethylene glycol), but their binding could be disrupted by pyridoxal 5'-phosphate; more than 70% recovery of the "activated" receptor complexes was achieved with buffer containing 20 mM pyridoxal 5'-phosphate. This interaction of "activated" glucocorticoid-receptor complexes of rat liver with histone-agarose suggests a role of histones in the mechanism of action of steroid hormone.
