暴露于马来西亚无刺蜂蜂蜜后生物膜的转录组分析
Transcriptome Analysis of Biofilm Following the Exposure to Malaysian Stingless Bee Honey.
作者信息
Seder Nesrin, Abu Bakar Mohd Hilmi, Abu Rayyan Walid Salem
机构信息
Faculty of Health Sciences, University Sultan Zain Al Abidin, Kuala Nerus, Terengganu 21300, Malaysia.
Department of Pharmacology and Biomedical Sciences, Faculty of Pharmacy, University of Petra, Amman, Jordan.
出版信息
Adv Appl Bioinform Chem. 2021 Jan 14;14:1-11. doi: 10.2147/AABC.S292143. eCollection 2021.
INTRODUCTION
Malaysian stingless bee honey () has been aroused as a potential antimicrobial compound with antibiofilm activity. The capability of the gram-negative bacillus to sustain a fatal infection is encoded in the bacterium genome.
METHODS
In the current study, a transcriptome investigation was performed to explore the mechanism underlying the biofilm dispersal of after the exposure to honey.
RESULTS
Microarray analysis of the biofilm treated by 20% honey has revealed a down-regulation of 3478 genes among the 6085 screened genes. Specifically, around 13.5% of the down-regulated genes were biofilm-associated genes. The mapping of the biofilm-associated pathways has shown an ultimate decrease in the expression levels of the D-GMP signaling pathway and diguanylate cyclases () genes responsible for c-di-GMP formation.
CONCLUSION
We predominantly report the lowering of c-di-GMP through the down-regulation of genes as the main mechanism of biofilm inhibition by honey.
引言
马来西亚无刺蜂蜂蜜已被视为一种具有抗生物膜活性的潜在抗菌化合物。革兰氏阴性杆菌维持致命感染的能力由细菌基因组编码。
方法
在本研究中，进行了转录组研究，以探索暴露于无刺蜂蜂蜜后铜绿假单胞菌生物膜分散的潜在机制。
结果
对用20%无刺蜂蜂蜜处理的铜绿假单胞菌生物膜进行微阵列分析，发现在6085个筛选基因中有3478个基因表达下调。具体而言，约13.5%的下调基因是生物膜相关基因。生物膜相关途径的图谱显示，负责环二鸟苷酸（c-di-GMP）形成的二鸟苷酸环化酶（DGCs）基因和D-GMP信号通路的表达水平最终降低。
结论
我们主要报道了通过下调DGCs基因降低c-di-GMP水平是无刺蜂蜂蜜抑制生物膜的主要机制。
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