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NR2F1-AS1/miR-140/己糖激酶2轴调控缺氧诱导的肝癌细胞糖酵解和迁移

NR2F1-AS1/miR-140/HK2 Axis Regulates Hypoxia-Induced Glycolysis and Migration in Hepatocellular Carcinoma.

作者信息

Li Xiao, Li Yize, Bai Shuang, Zhang Jing, Liu Zhengcai, Yang Jingyue

机构信息

Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xian 710032, People's Republic of China.

Department of Clinical Oncology, Xijing Hospital, Fourth Military Medical University, Xian 710032, People's Republic of China.

出版信息

Cancer Manag Res. 2021 Jan 15;13:427-437. doi: 10.2147/CMAR.S266797. eCollection 2021.

DOI:10.2147/CMAR.S266797
PMID:33488124
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7815091/
Abstract

BACKGROUND

Hypoxia is an important feature for the progression of hepatocellular carcinoma (HCC). Long noncoding RNA nuclear receptor subfamily 2 group F member 1 antisense RNA 1 (NR2F1-AS1) is dysregulated in HCC. However, the role and mechanism of N2RF1-AS1 in hypoxia-induced glycolysis and migration remain unclear.

MATERIALS AND METHODS

Tumor tissues and adjacent samples were harvested from 40 HCC patients. HCC cells were treated by hypoxia. The levels of NR2F1-AS1, microRNA (miR)-140, and hexokinase 2 (HK2) were examined via quantitative reverse transcription polymerase chain reaction or Western blot. Glycolysis was analyzed via glucose uptake, lactate production, and adenosine triphosphate (ATP) levels. Cell migration was analyzed via transwell assay. The target association was analyzed via dual-luciferase reporter assay and RNA immunoprecipitation.

RESULTS

NR2F1-AS1 level was enhanced in HCC tissues and cells. High expression of NR2F1-AS1 indicated poor overall survival. Silence of NR2F1-AS1 repressed hypoxia-induced glycolysis and migration in HCC cells. NR2F1-AS1 could regulate HK2 expression by modulating miR-140. miR-140 down-regulation or HK2 up-regulation mitigated the influence of NR2F1-AS1 silence on hypoxia-induced glycolysis and migration in HCC cells.

CONCLUSION

NR2F1-AS1 knockdown restrained hypoxia-induced glycolysis and migration in HCC cells via increasing miR-140 and decreasing HK2.

摘要

背景

缺氧是肝细胞癌（HCC）进展的一个重要特征。长链非编码RNA核受体亚家族2组F成员1反义RNA1（NR2F1-AS1）在HCC中表达失调。然而，N2RF1-AS1在缺氧诱导的糖酵解和迁移中的作用及机制仍不清楚。

材料与方法

收集40例HCC患者的肿瘤组织及癌旁组织样本。对HCC细胞进行缺氧处理。通过定量逆转录聚合酶链反应或蛋白质免疫印迹法检测NR2F1-AS1、微小RNA（miR）-140和己糖激酶2（HK2）的水平。通过葡萄糖摄取、乳酸生成和三磷酸腺苷（ATP）水平分析糖酵解情况。通过Transwell实验分析细胞迁移情况。通过双荧光素酶报告基因实验和RNA免疫沉淀分析靶标相关性。

结果

NR2F1-AS1水平在HCC组织和细胞中升高。NR2F1-AS1高表达提示总生存期较差。沉默NR2F1-AS1可抑制HCC细胞中缺氧诱导的糖酵解和迁移。NR2F1-AS1可通过调节miR-140来调控HK2的表达。miR-140下调或HK2上调可减轻NR2F1-AS1沉默对HCC细胞中缺氧诱导的糖酵解和迁移的影响。

结论

NR2F1-AS1基因敲低通过增加miR-140和降低HK2抑制HCC细胞中缺氧诱导的糖酵解和迁移。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5367/7815091/fd99e71568d0/CMAR-13-427-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5367/7815091/0d3a272a806b/CMAR-13-427-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5367/7815091/b587cb5da9ec/CMAR-13-427-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5367/7815091/6892011f90f3/CMAR-13-427-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5367/7815091/0d530246b8a7/CMAR-13-427-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5367/7815091/15dc4baca4ef/CMAR-13-427-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5367/7815091/fd99e71568d0/CMAR-13-427-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5367/7815091/0d3a272a806b/CMAR-13-427-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5367/7815091/b587cb5da9ec/CMAR-13-427-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5367/7815091/6892011f90f3/CMAR-13-427-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5367/7815091/0d530246b8a7/CMAR-13-427-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5367/7815091/15dc4baca4ef/CMAR-13-427-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5367/7815091/fd99e71568d0/CMAR-13-427-g0006.jpg

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2
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J Exp Clin Cancer Res. 2020 Jul 6;39(1):126. doi: 10.1186/s13046-020-01629-4.
3
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长链非编码RNA通过调控miR-512-3p/HK-2轴促进前列腺癌进展。
Asian J Urol. 2024 Oct;11(4):575-585. doi: 10.1016/j.ajur.2024.01.007. Epub 2024 Feb 2.
4
The regulation of hypoxia-related lncRNAs in hepatocellular carcinoma.肝细胞癌中缺氧相关长链非编码RNA的调控
Discov Oncol. 2024 May 7;15(1):144. doi: 10.1007/s12672-024-01002-3.
5
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Oncol Rep. 2024 Jun;51(6). doi: 10.3892/or.2024.8743. Epub 2024 Apr 26.
6
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7
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5
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6
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Biomed Res Int. 2019 Nov 7;2019:8983704. doi: 10.1155/2019/8983704. eCollection 2019.
7
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Aging (Albany NY). 2019 Dec 4;11(23):11609-11623. doi: 10.18632/aging.102563.
8
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9
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