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从生物膜菌落中提取重金属络合物用于生物监测污染。

Extraction of heavy metal complexes from a biofilm colony for biomonitoring the pollution.

作者信息

SÜrdem Sedat, DoĞan HacıMehmet

机构信息

National Boron Research Institute, Ankara Turkey.

Department of Chemistry, Faculty of Science, Hacettepe University, Ankara Turkey.

出版信息

Turk J Chem. 2020 Jun 1;44(3):712-725. doi: 10.3906/kim-1912-38. eCollection 2020.

DOI:10.3906/kim-1912-38
PMID:33488188
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7671218/
Abstract

An extraction method was tested for biomonitoring the biofilm samples containing heavy metals. The fractionation of metal complexes was performed via C-18-HPLC-ICP-MS and MALDI-MS, respectively. The extraction power of some reagents was determined for the heavy metal extraction from biofilm samples collected in Erdemli coast in the Mediterranean Sea. The ammonium acetate solution giving the highest extraction results was found as a suitable extraction reagent. The concentration and pH of the ammonium acetate solution were optimized and found as 1 M and 5, respectively. The chromatograms of metal complexes with the C-18-HPLC-ICP-MS system were taken to determine the effect of the pH of the metal complexes. After performing the extraction, metal bounded biomolecules were characterized by MALDI-MS for the fractions in the C18-HPLC system. It was seen that ammonium acetate extraction (1M, pH 5) might be used in biomonitoring studies due to relatively simple procedure, short analysis period, and low cost. The evaluation of the applicability of the method in biomonitoring studies might be supported by further studies with biofilms having similar characteristics.

摘要

测试了一种用于生物监测含重金属生物膜样品的提取方法。分别通过C-18高效液相色谱-电感耦合等离子体质谱（C-18-HPLC-ICP-MS）和基质辅助激光解吸电离质谱（MALDI-MS）对金属络合物进行分级分离。测定了一些试剂从地中海埃尔德姆利海岸采集的生物膜样品中提取重金属的能力。发现提取结果最高的醋酸铵溶液是一种合适的提取试剂。对醋酸铵溶液的浓度和pH值进行了优化，分别为1M和5。利用C-18-HPLC-ICP-MS系统采集金属络合物的色谱图，以确定金属络合物pH值的影响。提取后，通过MALDI-MS对C18-HPLC系统中的级分进行金属结合生物分子的表征。结果表明，醋酸铵提取法（1M，pH 5）操作相对简单、分析周期短、成本低，可用于生物监测研究。具有相似特征的生物膜的进一步研究可能有助于评估该方法在生物监测研究中的适用性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c26/7671218/3d89506a1be3/turkjchem-44-712-fig005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c26/7671218/e90e0897adcb/turkjchem-44-712-fig001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c26/7671218/66fba1f32e67/turkjchem-44-712-fig002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c26/7671218/f8d122e8995d/turkjchem-44-712-fig003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c26/7671218/c32aba4a53ff/turkjchem-44-712-fig004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c26/7671218/3d89506a1be3/turkjchem-44-712-fig005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c26/7671218/e90e0897adcb/turkjchem-44-712-fig001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c26/7671218/66fba1f32e67/turkjchem-44-712-fig002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c26/7671218/f8d122e8995d/turkjchem-44-712-fig003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c26/7671218/c32aba4a53ff/turkjchem-44-712-fig004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2c26/7671218/3d89506a1be3/turkjchem-44-712-fig005.jpg

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