YalÇin Bestenur, Akcan Doğan, YalÇin İbrahim Ertuğrul, Alphan Mehmet Can, ŞentÜrk Kenan, ÖzyİĞİt İbrahim İlker, Arda Lütfi
Department of Medical Laboratory Techniques, Vocational School of Health Services, Bahçeşehir University İstanbul Turkey.
Department of Mathematics Engineering, Faculty of Engineering and Natural Sciences, Bahçeşehir University, İstanbul Turkey.
Turk J Chem. 2020 Aug 18;44(4):1177-1199. doi: 10.3906/kim-2004-9. eCollection 2020.
The objective of this study is to synthesize ZnO and Mg doped ZnO (ZnMgO) nanoparticles via the sol-gel method, and characterize their structures and to investigate their biological properties such as antibacterial activity and hemolytic potential.Nanoparticles (NPs) were synthesized by the sol-gel method using zinc acetate dihydrate (Zn(CHCOO).2HO) and magnesium acetate tetrahydrate (Mg(CHCOO).4HO) as precursors. Methanol and monoethanolamine were used as solvent and sol stabilizer, respectively. Structural and morphological characterizations of ZnMgO nanoparticles were studied by using XRD and SEM-EDX, respectively. Photocatalytic activities of ZnO and selected Mg-doped ZnO (ZnMgO) nanoparticles were investigated by degradation of methylene blue (MeB). Results indicated that Mg doping (both 10% and 30%) to the ZnO nanoparticles enhanced the photocatalytic activity and a little amount of Zn0.90 Mg0.10 O photocatalyst (1.0 mg/mL) degraded MeB with 99% efficiency after 24 h of irradiation under ambient visible light. Antibacterial activity of nanoparticles versus ( ) was determined by the standard plate count method. Hemolytic activities of the NPs were studied by hemolysis tests using human erythrocytes. XRD data proved that the average particle size of nanoparticles was around 30 nm. Moreover, the XRD results indicatedthat the patterns of Mg doped ZnO nanoparticles related to ZnO hexagonal wurtzite structure had no secondary phase for x ≤ 0.2 concentration. For 0 ≤ x ≤ 0.02, NPs showed a concentration dependent antibacterial activity against . While ZnMg O totally inhibited the growth of , upper and lower dopant concentrations did not show antibacterial activity.
本研究的目的是通过溶胶 - 凝胶法合成氧化锌（ZnO）和镁掺杂氧化锌（ZnMgO）纳米颗粒，表征其结构，并研究它们的生物学特性，如抗菌活性和溶血潜力。使用二水合醋酸锌（Zn(CH₃COO)₂·2H₂O）和四水合醋酸镁（Mg(CH₃COO)₂·4H₂O）作为前驱体，通过溶胶 - 凝胶法合成纳米颗粒（NPs）。甲醇和单乙醇胺分别用作溶剂和溶胶稳定剂。分别使用X射线衍射（XRD）和扫描电子显微镜 - 能谱仪（SEM - EDX）研究了ZnMgO纳米颗粒的结构和形态特征。通过亚甲基蓝（MeB）的降解研究了ZnO和选定的镁掺杂氧化锌（ZnMgO）纳米颗粒的光催化活性。结果表明，向ZnO纳米颗粒中掺杂镁（10%和30%）均提高了光催化活性，在环境可见光照射24小时后，少量的Zn₀.₉₀Mg₀.₁₀O光催化剂（1.0 mg/mL）以99%的效率降解了MeB。通过标准平板计数法测定纳米颗粒对（此处原文缺失具体菌株信息）的抗菌活性。使用人红细胞通过溶血试验研究了NPs的溶血活性。XRD数据证明纳米颗粒的平均粒径约为30 nm。此外，XRD结果表明，对于x≤0.2的浓度，镁掺杂氧化锌纳米颗粒与ZnO六方纤锌矿结构相关的图谱没有第二相。对于0≤x≤0.02，NPs对（此处原文缺失具体菌株信息）表现出浓度依赖性抗菌活性。虽然ZnMgO完全抑制了（此处原文缺失具体菌株信息）的生长，但较高和较低的掺杂剂浓度均未显示出抗菌活性。
