Department of Animal Science, Visva Bharati University, Bolpur, India.
Department of LFC, West Bengal University of Animal and Fishery Sciences, Kolkata, India.
Front Immunol. 2021 Jan 8;11:534705. doi: 10.3389/fimmu.2020.534705. eCollection 2020.
Retinoic acid inducible gene I (RIG-I) is associated to the DExD/H box RNA helicases. It is a pattern recognition receptor (PRR), playing a crucial role in the system and is a germ line encoded host sensor to perceive pathogen-associated molecular patterns (PAMPs). So far, reports are available for the role of RIG-I in antiviral immunity. This is the first report in which we have documented the role of RIG-I in parasitic immunity. is a deadly parasite affecting the sheep industry, which has a tremendous economic importance, and the parasite is reported to be prevalent in the hot and humid agroclimatic region. We characterize the RIG-I gene in sheep () and identify the important domains or binding sites with through in silico studies. Differential mRNA expression analysis reveals upregulation of the RIG-I gene in the abomasum of infected sheep compared with that of healthy sheep, further confirming the findings. Thus, it is evident that, in infected sheep, expression of RIG-I is triggered for binding to more pathogens (). Genetically similar studies with humans and other livestock species were conducted to reveal that sheep may be efficiently using a model organism for studying the role of RIG-I in antiparasitic immunity in humans.
维甲酸诱导基因 I（RIG-I）与 DEAD/DEAH 盒 RNA 解旋酶相关。它是一种模式识别受体（PRR），在该系统中起着至关重要的作用，是一种胚系编码的宿主传感器，可感知病原体相关分子模式（PAMPs）。迄今为止，已有报道称 RIG-I 在抗病毒免疫中发挥作用。这是首次报道 RIG-I 在寄生虫免疫中的作用。 是一种致命的寄生虫，影响着绵羊产业，具有巨大的经济重要性，据报道，这种寄生虫在炎热潮湿的农业气候地区流行。我们在绵羊中表征了 RIG-I 基因，并通过计算机研究确定了与 的重要结构域或结合位点。差异 mRNA 表达分析显示，与健康绵羊相比，感染绵羊的胃脏中 RIG-I 基因上调，进一步证实了这一发现。因此，很明显，在感染的绵羊中，RIG-I 的表达被触发以与更多的病原体结合（）。对人类和其他牲畜进行了类似的遗传研究，以揭示绵羊可能是研究 RIG-I 在人类抗寄生虫免疫中的作用的有效模式生物。
