Department of BioChemistry, West Bengal University of Animal and Fishery Sciences, Kolkata, India.
Department of LFC, West Bengal University of Animal and Fishery Sciences, Kolkata, India.
Front Immunol. 2021 Jul 16;12:664877. doi: 10.3389/fimmu.2021.664877. eCollection 2021.
CD14 (also known as the monocyte differentiation antigen) is an important immune response gene known to be primarily responsible for innate immunity against bacterial pathogens, and as a pattern recognition receptor (PRR), binds with LPS (endotoxin), lipoproteins, and lipotechoic acid of bacteria. So far very limited work has been conducted in parasitic immunology. In the current study, we reported the role of CD14 in parasitic immunology in livestock species (sheep) for the first time. Ovine CD14 is characterized as a horse-shoe shaped bent solenoid with a hydrophobic amino-terminal pocket for CD14 along with domains. High mutation frequency was observed, out of total 41 mutations identified, 23 mutations were observed to be thermodynamically unstable and 11 mutations were deleterious in nature, causing major functional alteration of important domains of CD14, an indication of variations in individual susceptibility for sheep against infestations. studies with molecular docking reveal a role of immune response against in sheep, which is later confirmed with experimental evidence through differential mRNA expression analysis for sheep, which revealed better expression of CD14 in infected sheep compared to that of non-infected sheep. We confirmed the above findings with supportive evidence through haematological and biochemical analyses. Phylogenetic analysis was conducted to assess the evolutionary relationship with respect to humans and it was observed that sheep may well be used as model organisms due to better genetic closeness compared to that of mice.
CD14（也称为单核细胞分化抗原）是一个重要的免疫反应基因，主要负责先天免疫对抗细菌病原体，作为模式识别受体（PRR），与 LPS（内毒素）、脂蛋白和细菌的脂多糖结合。到目前为止，寄生虫免疫学的研究非常有限。在本研究中，我们首次报道了 CD14 在家畜（绵羊）寄生虫免疫学中的作用。绵羊 CD14 的特征是马蹄形弯曲螺线管，带有疏水的氨基末端口袋，用于 CD14 以及结构域。观察到高突变频率，在总共鉴定的 41 个突变中，有 23 个突变被观察到热力学不稳定，11 个突变具有破坏性，导致 CD14 重要结构域的主要功能改变，表明个体对绵羊感染的易感性存在差异。分子对接研究表明 CD14 在绵羊对 感染的免疫反应中发挥作用，这一发现后来通过绵羊的差异 mRNA 表达分析得到了实验证据的证实，与未感染的绵羊相比，感染绵羊的 CD14 表达更好。我们通过血液学和生物化学分析提供了支持性证据来证实上述发现。进行了系统发育分析以评估与人类的进化关系，观察到由于与小鼠相比遗传上更接近，绵羊可能被很好地用作模型生物。
