Xu Zhe, Liu Xiaoguang, Li Yanqing, Gao Hongliang, He Tao, Zhang Chunlei, Hao Wei, Teng Xu
Department of Anesthesiology, Hebei Provincial Hospital of Traditional Chinese Medicine, Shijiazhuang, Hebei 050011, P.R. China.
Department of Gynecology, Hebei Provincial Hospital of Traditional Chinese Medicine, Shijiazhuang, Hebei 050011, P.R. China.
Exp Ther Med. 2021 Mar;21(3):197. doi: 10.3892/etm.2021.9630. Epub 2021 Jan 8.
Osteoporosis (OP) and vascular calcification (VC) share a number of common risk factors, pathophysiological mechanisms and etiology, which are known as bone-vascular axis. The present study aimed to investigate the effects of Shuxuetong (SXT) injection on VC and osteoporosis. A rat model of VC and osteoporosis was induced by dexamethasone (DEX; 1 mg/kg/day for 4 weeks, intramuscularly). Simultaneously, 0.6 ml/kg/day SXT was intraperitoneally injected. Compared with control rats, DEX induced significantly more VC and OP, as determined by increased calcium deposition and alkaline phosphatase activity in the aorta, disturbed structure, decreased levels of cortical bone thickness and trabecular bone area, and increased apoptosis in the bone. SXT injection ameliorated DEX-induced VC and osteoporosis; furthermore, the osteoblastic differentiation of vascular smooth muscle cells and the activation of endoplasmic reticulum stress in the DEX group was also prevented by SXT injection. Compared with control rats, protein expression levels of sclerostin, a crucial crosslink of the bone-vascular axis, were significantly increased in the aorta and bone of rats with DEX, which was also attenuated by SXT injection. Thus, the present study suggested that SXT injection could ameliorate both VC and OP, and may be mediated by the regulation of sclerostin. The present study may provide the basis a novel strategy for the prevention and treatment of VC and OP, which emerge as side-effects of glucocorticoids.
骨质疏松症（OP）与血管钙化（VC）具有许多共同的危险因素、病理生理机制和病因，这被称为骨-血管轴。本研究旨在探讨舒血宁（SXT）注射液对VC和骨质疏松症的影响。通过地塞米松（DEX；1mg/kg/天，肌肉注射，共4周）诱导建立VC和骨质疏松症大鼠模型。同时，腹腔注射0.6ml/kg/天的SXT。与对照大鼠相比，DEX诱导了更多的VC和OP，这通过主动脉中钙沉积增加和碱性磷酸酶活性升高、结构紊乱、皮质骨厚度和小梁骨面积水平降低以及骨细胞凋亡增加来确定。SXT注射改善了DEX诱导的VC和骨质疏松症；此外，SXT注射还预防了DEX组血管平滑肌细胞的成骨分化和内质网应激的激活。与对照大鼠相比，骨-血管轴的关键交联蛋白硬化蛋白在DEX大鼠的主动脉和骨骼中的蛋白表达水平显著升高，而SXT注射也使其减弱。因此，本研究表明SXT注射可以改善VC和OP，并且可能通过对硬化蛋白的调节来介导。本研究可能为作为糖皮质激素副作用出现的VC和OP的预防和治疗提供一种新策略的依据。
