Pohlentz G, Klein D, Schmitz D, Schwarzmann G, Peter-Katalinić J, Sandhoff K
Institut für Organische Chemie und Biochemie, Universität Bonn.
Biol Chem Hoppe Seyler. 1988 Jan;369(1):55-63. doi: 10.1515/bchm3.1988.369.1.55.
Biosynthesis of glycolipids GA2, GA1, GM1b, and GD1c was studied in Golgi vesicles isolated from rat liver. Sequential addition of N-acetylgalactosamine, galactose and two sialic acid residues to lactosylceramide led to the endproduct GD1c. Activities of the corresponding glycosyltransferases were shown to be present in isolated Golgi vesicles and their respective kinetic data were determined. The products of each reaction were characterized by their mobility on thin-layer chromatography, by enzymic degradation to their respective precursors, and in case of GM1b by FAB mass spectrometry.
在从大鼠肝脏分离出的高尔基体囊泡中研究了糖脂GA2、GA1、GM1b和GD1c的生物合成。向乳糖基神经酰胺依次添加N-乙酰半乳糖胺、半乳糖和两个唾液酸残基，生成终产物GD1c。结果表明，相应的糖基转移酶活性存在于分离出的高尔基体囊泡中，并测定了它们各自的动力学数据。每个反应的产物通过其在薄层色谱上的迁移率、酶促降解为各自的前体以及对于GM1b通过快原子轰击质谱进行表征。
