Yohem K H, Slymen D J, Bregman M D, Meyskens F L
Arizona Cancer Center, University of Arizona Health Sciences Center, Tucson 85724.
Br J Cancer. 1988 Jan;57(1):64-9. doi: 10.1038/bjc.1988.10.
The radiation response of murine and human melanoma cells assayed in bilayer soft agar and monolayer was examined. Cells from the murine melanoma Cloudman S91 CCL 53.1 cell line and three human melanoma cell strains (C8146C, C8161, and R83-4) developed in our laboratory were irradiated by single dose X-rays and plated either in agar or on plastic. D0 values were the same within 95% confidence intervals for cells from the human melanoma cell strains C8146C, C8161, and R83-4 but were dissimilar for the murine cell line CCL 53.1 Dq values were different for all cells studied. The shape of the survival curve for all four melanomas was not identical for cells assayed in soft agar versus cells grown on plastic. This would indicate that apparent radiosensitivity was influenced by the method of assay although there were no apparent consistent differences between the curves generated by monolayer or bilayer soft agar assays.
研究了在双层软琼脂和单层培养中检测的小鼠和人类黑色素瘤细胞的辐射反应。来自小鼠黑色素瘤Cloudman S91 CCL 53.1细胞系的细胞以及我们实验室培养的三个人类黑色素瘤细胞株(C8146C、C8161和R83 - 4)接受单剂量X射线照射后,接种于琼脂或塑料培养皿上。对于人类黑色素瘤细胞株C8146C、C8161和R83 - 4的细胞,D0值在95%置信区间内相同,但对于小鼠细胞系CCL 53.1则不同。所有研究细胞的Dq值都不同。在软琼脂中检测的细胞与在塑料上生长的细胞相比,所有四种黑色素瘤的存活曲线形状并不相同。这表明表观放射敏感性受检测方法的影响,尽管单层或双层软琼脂检测所产生的曲线之间没有明显一致的差异。