Amin Mohsin, Preuss Andrea, Deisenroth Ted, Liauw Christopher M, Verran Joanna, Whitehead Kathryn A
Microbiology at Interfaces, Manchester Metropolitan University, Chester St, Manchester M1 5GD, UK.
Research Centre, Germany.
iScience. 2020 Dec 19;24(1):101962. doi: 10.1016/j.isci.2020.101962. eCollection 2021 Jan 22.
Binding of particles and spores to surfaces is a natural phenomenon which is a prerequisite for biofilm formation. Perpendicular force measurements were carried out using atomic force microscopy cantilevers modified with a polystyrene or glass sphere. The attachment of the spheres was tested against glass, PVAc, p(γ-MPSco-MMA), p(γ-MPS-co-LMA), PMMAsc, and silicon surfaces. The polystyrene spheres demonstrated less varied force and strength of attachment measurement to the surfaces than the glass spheres. The force of attachment of the polystyrene spheres was also influenced by mobility of the co-polymer surfaces. Surface wettability did not affect the force of polystyrene or glass sphere attachment. The force measurements of the non-biological spheres were similar to those seen in biological systems with fungal conidia, and this was due to their size, shape, and binding energies. The use of non-biological systems may present an insight into understanding the fundamentals of more complex biological processes.
颗粒和孢子与表面的结合是一种自然现象，是生物膜形成的先决条件。使用用聚苯乙烯或玻璃球修饰的原子力显微镜悬臂进行垂直力测量。测试了这些球体在玻璃、聚醋酸乙烯酯、聚（γ-甲基丙烯酰氧基丙基三甲氧基硅烷-co-甲基丙烯酸甲酯）、聚（γ-甲基丙烯酰氧基丙基三甲氧基硅烷-co-月桂酸甲酯）、聚甲基丙烯酸甲酯共聚物和硅表面上的附着情况。与玻璃球相比，聚苯乙烯球对表面的附着力测量结果在力和强度方面变化较小。聚苯乙烯球的附着力还受到共聚物表面流动性的影响。表面润湿性不影响聚苯乙烯或玻璃球的附着力。非生物球体的力测量结果与真菌分生孢子的生物系统中的测量结果相似，这是由于它们的大小、形状和结合能。使用非生物系统可能有助于深入了解更复杂生物过程的基本原理。
