Santucci R, Mintorovitch J, Constantinidis I, Satterlee J D, Ascoli F
Department of Biochemical Sciences, University La Sapienza, Rome, Italy.
Biochim Biophys Acta. 1988 Mar 23;953(2):201-4. doi: 10.1016/0167-4838(88)90025-8.
Circular dichroism spectra of three monomeric components of Glycera dibranchiata hemoglobins are reported. Contrary to what is found for most hemoglobins and myoglobins, G. dibranchiata hemoglobins display largely negative dichroic spectra in the Soret region. Independent NMR measurements have shown that the same monomeric hemoglobin components contain the heme moiety predominantly (greater than 85%) oriented in a reversed way with respect to the orientation which occurs in most hemoglobins and myoglobins. On the basis of these independent NMR studies, and also of previous data on other invertebrate hemoproteins, a correlation appears evident between reversed heme orientation in hemoglobins and negative ellipticity in the Soret CD spectrum. This represents a simple tool to evaluate this aspect of heme asymmetric environment.
报道了双分支甘油血红蛋白三种单体成分的圆二色光谱。与大多数血红蛋白和肌红蛋白不同，双分支甘油血红蛋白在索雷特区域呈现出大部分为负的二色光谱。独立的核磁共振测量表明，相同的单体血红蛋白成分含有主要（大于85%）以与大多数血红蛋白和肌红蛋白中出现的方向相反的方式取向的血红素部分。基于这些独立的核磁共振研究以及先前关于其他无脊椎动物血红蛋白的数据，血红蛋白中血红素反向取向与索雷特圆二色光谱中的负椭圆率之间的相关性似乎很明显。这是评估血红素不对称环境这一方面的一个简单工具。
