从老鼠睾丸中分离精母细胞的方案。
Protocol for isolation of spermatids from mouse testes.
机构信息
Research Center for Epigenetic Code and Diseases, Department of Biological Sciences, Seoul National University, Seoul 08826, South Korea.
Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan.
出版信息
STAR Protoc. 2021 Jan 8;2(1):100254. doi: 10.1016/j.xpro.2020.100254. eCollection 2021 Mar 19.
Post-meiotic spermatids become spermatozoa through developmental stages during spermiogenesis. Isolation of spermatid fractions is required to examine the change of protein expression during spermiogenesis. Here, we present a simple method to isolate spermatid fractions from mouse testes using unit gravity sedimentation in a BSA density gradient. Isolation of spermatid fractions can be used to analyze changes of transcript or protein during spermiogenesis. For complete details on the use and execution of this protocol, please refer to Kim et al. (2020).
减数分裂后的精母细胞通过精子发生过程中的发育阶段成为精子。为了研究精子发生过程中蛋白质表达的变化，需要分离精母细胞的各个部分。这里，我们使用 BSA 密度梯度的单位重力沉降法，提出了一种从小鼠睾丸中分离精母细胞部分的简单方法。精母细胞部分的分离可用于分析精子发生过程中转录本或蛋白质的变化。如需详细了解本方案的使用和执行，请参考 Kim 等人（2020 年）的研究。
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