一种从多能干细胞中生成乙型肝炎病毒病毒抗原特异性 T 淋巴细胞的优化方案。
An optimized protocol for the generation of HBV viral antigen-specific T lymphocytes from pluripotent stem cells.
机构信息
Department of Microbial Pathogenesis and Immunology, Texas A&M University Health Science Center, MREB II, Room 3344, 8447 Riverside Pkwy, Bryan, TX 77807, USA.
Department of Ophthalmology, Harvard Medical School, Boston, MA 02215, USA.
出版信息
STAR Protoc. 2021 Jan 12;2(1):100264. doi: 10.1016/j.xpro.2020.100264. eCollection 2021 Mar 19.
In T cell-based cancer immunotherapy, tumor antigen (Ag)-specific CD8 cytotoxic T lymphocytes (CTLs) can specifically target tumor Ags on malignant cells. This promising approach drove us to adopt this strategy of T cell transfer (ACT)-based immunotherapy for chronic viral infections. Here, we describe the generation of hepatitis B virus (HBV) Ag-specific CTLs from induced pluripotent stem cells (iPSCs), i.e., iPSC-CTLs. Ag-specific iPSC-CTLs can target HBV Ag cells and infiltrate into the liver to suppress HBV replication in a murine model. For complete details on the use and execution of this protocol, please refer to Haque et al. (2020).
在基于 T 细胞的癌症免疫疗法中，肿瘤抗原（Ag）特异性 CD8 细胞毒性 T 淋巴细胞（CTL）可以特异性地针对恶性细胞上的肿瘤 Ag。这种有前途的方法促使我们采用基于 T 细胞转移（ACT）的免疫疗法来治疗慢性病毒感染。在这里，我们描述了从诱导多能干细胞（iPSC）中产生乙型肝炎病毒（HBV）Ag 特异性 CTL，即 iPSC-CTL。Ag 特异性 iPSC-CTL 可以靶向 HBV Ag 细胞并浸润肝脏，从而在小鼠模型中抑制 HBV 复制。有关此方案使用和执行的完整详细信息，请参阅 Haque 等人。（2020 年）。
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