Division of Life Science, State Key Laboratory of Molecular Neuroscience, Molecular Neuroscience Center, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China.
Hong Kong Center for Neurodegenerative Diseases, Hong Kong, China.
STAR Protoc. 2021 Jan 13;2(1):100265. doi: 10.1016/j.xpro.2020.100265. eCollection 2021 Mar 19.
Alzheimer's disease is characterized by the deposition of extracellular amyloid-beta (Aβ) plaques. While microglial phagocytosis is a major mechanism through which Aβ is cleared, there is no method for quantitatively assessing Aβ phagocytic capacity of microglia . Here, we present a flow cytometry-based method for investigating the Aβ phagocytic capacity of microglia . This method enables the direct comparison of Aβ phagocytic capacity between different microglial subpopulations as well as the direct isolation of Aβ phagocytic microglia for downstream applications. For complete details on the use and execution of this protocol, please refer to Lau et al. (2020).
阿尔茨海默病的特征是细胞外淀粉样蛋白-β（Aβ）斑块的沉积。虽然小胶质细胞吞噬是清除 Aβ 的主要机制，但目前还没有定量评估小胶质细胞 Aβ吞噬能力的方法。在这里，我们提出了一种基于流式细胞术的方法来研究小胶质细胞的 Aβ吞噬能力。该方法能够直接比较不同小胶质细胞亚群之间的 Aβ吞噬能力，并且能够直接分离 Aβ吞噬小胶质细胞用于下游应用。有关该方案的使用和执行的完整详细信息，请参考 Lau 等人（2020 年）。
