INSERM UMR 1186, Integrative Tumor Immunology and Immunotherapy, Gustave Roussy, Fac. de Médecine - Univ. Paris-Sud, Université Paris-Saclay, Villejuif 94805, France.
CNRS ums3655 INSERM us23 Gustave Roussy Cancer Campus, Villejuif 94805, France.
STAR Protoc. 2021 Jan 12;2(1):100267. doi: 10.1016/j.xpro.2020.100267. eCollection 2021 Mar 19.
CD103CD8 tumor-resident memory T cells (T) are important components of anti-tumor immunity. However, their role in response to cancer immunotherapy is not fully understood. The protocol describes how to isolate CD8 T cells and autologous tumor cells from human lung tumors to study the functional activities of CD8 T cells. Tumors are heterogeneous in terms of the quantity and quality of immune cell types, so the yield of T cells depends on the features of the tumor. For complete details on the use and execution of this protocol, please refer to Corgnac et al. (2020).
CD103CD8 肿瘤驻留记忆 T 细胞（T）是抗肿瘤免疫的重要组成部分。然而，它们在癌症免疫治疗中的作用尚未完全阐明。本方案描述了如何从人肺肿瘤中分离 CD8 T 细胞和自体肿瘤细胞，以研究 CD8 T 细胞的功能活性。肿瘤在免疫细胞类型的数量和质量方面存在异质性，因此 T 细胞的产量取决于肿瘤的特征。有关使用和执行本方案的完整详细信息，请参考 Corgnac 等人。（2020 年）。
