Isolation of tumor-resident CD8 T cells from human lung tumors.

CD103CD8 tumor-resident memory T cells (T) are important components of anti-tumor immunity. However, their role in response to cancer immunotherapy is not fully understood. The protocol describes how to isolate CD8 T cells and autologous tumor cells from human lung tumors to study the functional activities of CD8 T cells. Tumors are heterogeneous in terms of the quantity and quality of immune cell types, so the yield of T cells depends on the features of the tumor. For complete details on the use and execution of this protocol, please refer to Corgnac et al. (2020).