Ovalioglu Talat Cem, Canaz Gokhan, Arslan Sema, Ovalioglu Aysegul Ozdemir, Sar Mehmet, Aydin Ayşegül Esen, Emel Erhan
Bakirkoy Research and Training Hospital for Neurology, Neurosurgery and Psychiatry, Department of Neurosurgery, Istanbul, Turkey.
Turk Neurosurg. 2021;31(1):137-141. doi: 10.5137/1019-5149.JTN.32887-20.1.
To investigate the effects of quetiapine exposure on neural tube development in early stage chicken embryos.
Eighty-four fertilised specific pathogen-free chicken eggs were divided into four equal groups (groups 1?4). Three experimental groups (groups 2, 3 and 4) and a single control group (group 1) were used. Each egg in group 2 (n=21) was injected with 20 ?L of saline after 30 hours of incubation. Eggs in groups 3 and 4 were injected with 0.02 ml of a solution containing 400 and 800 ?g of quetiapine dose, respectively. Incubation was continued until the end of 72 hours. All embryos were then removed from the eggs and histopathologically examined.
Normal development and the closed neural tubes were shown in 18, 16, 13 and 9 embryos in groups 1 2, 3 and 9, respectively, of the 84 embryos incubated. Open neural tubes were found in one, three and five embryos in groups 2, 3 and 5, respectively. Also, developmental anomalies were found in three, four, five and seven embryos in groups 1, 2, 3 and 4, respectively. Moreover, no significant relationship between NTD and quetiapine exposure had been found.
Quetiapine has no significant effect on the occurrence of neural tube defects in the chicken embryo model.
研究喹硫平暴露对鸡胚早期神经管发育的影响。
84枚受精的无特定病原体鸡卵被分为四组（第1 - 4组），包括三个实验组（第2、3、4组）和一个对照组（第1组）。第2组（n = 21）的每枚鸡蛋在孵化30小时后注射20μL生理盐水。第3组和第4组的鸡蛋分别注射0.02 ml含400μg和800μg喹硫平剂量的溶液。孵化持续至72小时结束。然后将所有胚胎从鸡蛋中取出并进行组织病理学检查。
在孵化的84枚胚胎中，第1、2、3和4组分别有18、16、13和9枚胚胎发育正常且神经管闭合。第2、3和4组分别有1、3和5枚胚胎出现神经管开放。此外，第1、2、3和4组分别有3、4、5和7枚胚胎出现发育异常。而且，未发现神经管缺陷与喹硫平暴露之间存在显著关系。
在鸡胚模型中，喹硫平对神经管缺陷的发生没有显著影响。
