Veroman S A
Biull Eksp Biol Med. 1988 Feb;105(2):237-40.
144 crystalline lenses of swine fetuses, 10 to 20 Mm long, were organ-cultured for up to 42 days in Eagles medium or DMEM in combination with 6% or 20% fetal calf serum. The types of cells detected included epithelioid and infiltrating cells (in 11.1% of lenses), fibroblast-like cells (in 20.8% of lenses) and vesicular cells (in 49.3% of lenses). These cell types characteristic of pathological (cataract-affected) lenses in vivo are absent in normal lenses. According to the data obtained, long-term organ culture of the fetal eye lens may be used as an experimental model of cataractogenesis.
144个长度为10至20毫米的猪胎儿晶状体在含有6%或20%胎牛血清的伊格尔培养基或杜氏改良伊格尔培养基中进行器官培养长达42天。检测到的细胞类型包括上皮样细胞和浸润细胞(占晶状体的11.1%)、成纤维细胞样细胞(占晶状体的20.8%)和囊泡细胞(占晶状体的49.3%)。这些细胞类型是体内病理性(受白内障影响)晶状体所特有的,正常晶状体中不存在。根据所获得的数据,胎儿眼晶状体的长期器官培养可用作白内障发生的实验模型。