Department of Environmental Engineering, Bolu Abant Izzet Baysal University, Golkoy Campus, Bolu 14030, Turkey.
Biodesign Swette Center for Environmental Biotechnology, Arizona State University, Tempe, AZ 85287, USA.
Sci Total Environ. 2021 May 15;769:145217. doi: 10.1016/j.scitotenv.2021.145217. Epub 2021 Jan 18.
Globally, the demand for sustainable energy production and high-value biological compounds have become intertwined in an attempt to improve the feasibility of sustainable algal cultivation. Marine microalgae, especially diatoms and coccolithophores, represent viable cultures that can produce biofuels and high-value compounds. Growing them in co-culture offers the potential to produce lipids and pigments, while also generating CaCO for C sequestration. The main objective of this work was to investigate competition or co-existence of the diatom Chaetoceros gracilis and the coccolithophore Pleurochrysis Carterae. The focus was on the effects of silicate and co-culturing on the growth rate, productivity, pigment production, and ash production for C. gracilis and P. carterae in laboratory conditions. The results showed that, in monoculture, 2-mM Si enhanced the specific growth rate of C. gracilis, but did not affect P. carterae. Regardless of silicate concentration, C. gracilis was more productive than P. carterae. In co-culture, P. carterae had a slower growth rate, indicating an inhibitory effect of C. gracilis on P. carterae. Neither silicate concentration nor co-culturing had an impact on the contents of pigments fucoxanthin, chlorophyll-a, and chlorophyll-c, which means that pigment productivity was proportional to biomass productivity. Finally, the ash content increased in all cultures with the lower silicate concentration (0.2 mM) in the medium. With one exception, the ash content was dominated by SiO regardless of silicate amount, and CaCO was a major part of the ash only when P. carterae was grown separately with the higher silicate level. These results highlight that co-culturing did not provide an advantage for improving biomass, pigments, or CaCO productivity.
全球范围内，对可持续能源生产和高价值生物化合物的需求已经交织在一起，试图提高可持续藻类培养的可行性。海洋微藻，特别是硅藻和颗石藻，是可行的培养物，可以生产生物燃料和高价值化合物。在共培养中培养它们有潜力产生脂质和色素，同时也为 C 封存生成 CaCO3。这项工作的主要目的是研究硅藻 Chaetoceros gracilis 和颗石藻 Pleurochrysis Carterae 的竞争或共存。重点是研究硅酸盐和共培养对 C. gracilis 和 P. carterae 在实验室条件下的生长速率、生产力、色素产生和灰分产生的影响。结果表明，在单培养中，2-mM Si 提高了 C. gracilis 的比生长速率，但对 P. carterae 没有影响。无论硅酸盐浓度如何，C. gracilis 的生产力都高于 P. carterae。在共培养中，P. carterae 的生长速度较慢，表明 C. gracilis 对 P. carterae 有抑制作用。硅酸盐浓度和共培养都没有影响叶黄素、叶绿素-a 和叶绿素-c 的含量，这意味着色素生产力与生物质生产力成正比。最后，所有培养物中的灰分含量都随着培养基中较低硅酸盐浓度（0.2 mM）的增加而增加。除一个例外，无论硅酸盐的量如何，灰分主要由 SiO 组成，只有当 P. carterae 在较高硅酸盐水平下单独生长时，CaCO3 才是灰分的主要部分。这些结果表明，共培养并没有为提高生物质、色素或 CaCO3 生产力提供优势。
