Kraker A J, Moore C W
Department of Chemotherapy, Warner-Lambert/Parke-Davis Pharmaceutical Research, Ann Arbor, MI 48105.
Cancer Lett. 1988 Jan;38(3):307-14. doi: 10.1016/0304-3835(88)90022-5.
The activity of DNA polymerase beta, which is an enzyme involved in repair of DNA damage, was assessed in P388 murine leukemia cell lines sensitive and resistant to cis-diamminedichloroplatinum(II) (cis-Pt). The resistant line was selected with cis-Pt and showed cross-resistance to a number of alkylating agents, but not to an anthracycline. The DNA polymerase beta activity was found to be elevated 5-fold in the resistant line based upon activity per mg cell protein and elevated 4-fold based upon activity per 10(7) cells. The characterization of elevated activity of an enzyme active in DNA repair in a cell line resistant to DNA damaging agents describes a possible mechanism of resistance in addition to those previously found.
