L-2,4-二氨基丁酸对恶性胶质瘤细胞和成纤维细胞的细胞溶解作用。

The cytolytic effect of L-2,4 diaminobutyric acid with malignant glioma cells and fibroblasts.

作者信息

Panasci L, McQuillan A, Feindel W

机构信息

Lady Davis Institute, Montreal, Quebec, Canada.

出版信息

Cancer Chemother Pharmacol. 1988;21(2):143-4. doi: 10.1007/BF00257361.

DOI:10.1007/BF00257361

PMID:3349563

Abstract

L-2,4-Diaminobutyric acid (DABA), an amino acid analogue, produced a cytolytic effect with a human glioma cell line, SKMG-1, and normal human fibroblasts. The concentrations of DABA necessary to reduce the cell count to 50% of control (LD50) following a 24-h incubation at 37 degrees C were 12.5 mM for the human fibroblasts and 20 mM for the glioma cell line. The concentrations of DABA necessary to produce an LD50 after a 48-h incubation at 37 degrees C were 10 mM for the human fibroblasts and 14 mM for the human glioma cell line. The cytolytic effect of DABA was similar in the absence or the presence of serum with the human glioma cell line. The cytolytic effect of 20 mM DABA was partially prevented by the presence of 5 mM methyl-AIB. DABA was not preferentially toxic to this human glioma cell line compared with normal fibroblasts.

摘要

L-2,4-二氨基丁酸（DABA），一种氨基酸类似物，对人胶质瘤细胞系SKMG-1和正常人成纤维细胞产生了细胞溶解作用。在37℃孵育24小时后，将细胞计数降至对照的50%（LD50）所需的DABA浓度，人成纤维细胞为12.5 mM，胶质瘤细胞系为20 mM。在37℃孵育48小时后产生LD50所需的DABA浓度，人成纤维细胞为10 mM，人胶质瘤细胞系为14 mM。DABA对人胶质瘤细胞系的细胞溶解作用在有无血清的情况下相似。5 mM甲基-AIB的存在部分阻止了20 mM DABA的细胞溶解作用。与正常成纤维细胞相比，DABA对这种人胶质瘤细胞系没有优先毒性。