Beaubier Sophie, Przybylski Rémi, Bodin Alice, Nedjar Naïma, Dhulster Pascal, Kapel Romain
Laboratoire Réactions et Génie des Procédés, Université de Lorraine, UMR CNRS 7274, LRGP, F-54500 Vandœuvre-lès-Nancy, France.
University Lille, INRA, ISA, University Artois, University Littoral Côte d'Opale, EA 7394-ICV-Charles Viollette Institute, F-59000 Lille, France.
Membranes (Basel). 2021 Jan 20;11(2):73. doi: 10.3390/membranes11020073.
Hydrolysis of bovine hemoglobin (bHb), the main constituent of bovine cruor by-product, releases a natural antimicrobial peptide (NKT) which could present a major interest for food safety. To enrich this, tangential ultrafiltration can be implemented, but ultrafiltration conditions are mainly empirically established. In this context, the application of a simulation method for predicting the NKT yield and enrichment was investigated. Ultrafiltration performances were studied for decolored bHb hydrolysates at different degrees of hydrolysis (DH; 3%, 5%, 10% and 18%) and colored hydrolysates (3% and 5% DH) with 1 and 3 kg·mol regenerated cellulose membranes. The simulation method helped to identify the most promising hydrolysate (in terms of NKT enrichment, yield and productivity) as the 3% DH colored hydrolysate, and UF conditions (volumetric reduction factor of 5 and 3 with 1 and 3 kg·mol membrane, respectively) for higher antimicrobial recovery. A maximal enrichment factor of about 29 and NKT purity of 70% in permeate were observed. The results showed that the antimicrobial activity was in relation with the process selectivity and NKT purity. Finally, this reliable method, applied for predicting the ultrafiltration performances to enrich peptides of interest, is part of a global approach to rationally valorize protein resources from various by-products.
牛血红蛋白（bHb）是牛凝血副产物的主要成分，其水解会释放出一种天然抗菌肽（NKT），这可能对食品安全具有重大意义。为了富集这种抗菌肽，可以采用切向超滤，但超滤条件主要是凭经验确定的。在此背景下，研究了一种用于预测NKT产量和富集效果的模拟方法的应用。使用1和3 kg·mol的再生纤维素膜，对不同水解度（DH；3%、5%、10%和18%）的脱色bHb水解产物以及3%和5% DH的有色水解产物的超滤性能进行了研究。该模拟方法有助于确定最有前景的水解产物（就NKT富集、产量和生产率而言）为3% DH的有色水解产物，以及有助于提高抗菌剂回收率的超滤条件（分别使用1和3 kg·mol膜时，体积减少因子为5和3）。在渗透物中观察到最大富集因子约为29，NKT纯度为70%。结果表明，抗菌活性与过程选择性和NKT纯度有关。最后，这种用于预测超滤性能以富集目标肽的可靠方法，是合理利用各种副产物中蛋白质资源的整体方法的一部分。
