微小 RNA-186 和 655 调控垂体肿瘤转化基因 1 的改变调节人口腔鳞状细胞癌细胞的侵袭能力。
Alteration of Pituitary Tumor Transforming Gene 1 by MicroRNA-186 and 655 Regulates Invasion Ability of Human Oral Squamous Cell Carcinoma.
机构信息
Department of Oral Pathology, College of Dentistry, Gangneung-Wonju National University, Gangneung 25457, Korea.
Department of Biomedical Science, CHA University, Seoul 13488, Korea.
出版信息
Int J Mol Sci. 2021 Jan 20;22(3):1021. doi: 10.3390/ijms22031021.
BACKGROUND
Pituitary tumor-transforming gene 1 (PTTG1) was recently shown to be involved in the progression as well as the metastasis of cancers. However, their expression and function in the invasion of oral squamous cell carcinoma (SCC) remain unclear.
METHODS
The expressions of PTTG1 and PTTG1-targeted miRNA in oral SCC cell lines and their invasion capability depended on PTTG1 expression were analyzed by quantitative RT-PCR, Western blots, the transwell insert system and Zymography.
RESULTS
Invasion abilities were decreased in oral SCC cells treated with siRNA-PTTG1. When PTTG1 were downregulated in oral SCC cells treated with microRNA-186 and -655 inhibited their invasion abilities via MMP-9 activity.
CONCLUSIONS
These results indicate that alteration of expression of PTTG1 in oral SCC cells by newly identified microRNA-186 and -655 can regulate invasion activity. Therefore, these data offer new insights into further understanding PTTG1 function in oral SCC and should provide new strategies for diagnostic markers for oral SCC.
背景
垂体肿瘤转化基因 1（PTTG1）最近被证明参与了癌症的进展和转移。然而，它们在口腔鳞状细胞癌（SCC）侵袭中的表达和功能仍不清楚。
方法
通过定量 RT-PCR、Western blot、Transwell 插入系统和 Zymography 分析口腔 SCC 细胞系中 PTTG1 和 PTTG1 靶向 miRNA 的表达及其依赖 PTTG1 表达的侵袭能力。
结果
用 siRNA-PTTG1 处理口腔 SCC 细胞可降低其侵袭能力。当口腔 SCC 细胞中的 PTTG1 被下调时，miR-186 和 miR-655 通过 MMP-9 活性抑制其侵袭能力。
结论
这些结果表明，新鉴定的 microRNA-186 和 -655 改变口腔 SCC 细胞中 PTTG1 的表达可以调节侵袭活性。因此，这些数据为进一步了解 PTTG1 在口腔 SCC 中的功能提供了新的见解，并应为口腔 SCC 的诊断标志物提供新的策略。
Zotero 插件