College of Agriculture, Guangxi University, Nanning 530006, China.
Maize Research Institute of Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
Int J Mol Sci. 2021 Jan 23;22(3):1107. doi: 10.3390/ijms22031107.
Male sterility (MS) plays a key role in the hybrid breed production of plants. Researchers have focused on the association between genetic male sterility (GMS) and cytoplasmic male sterility (CMS) in kenaf. In this study, P9BS (a natural GMS mutant of the kenaf line P9B) and male plants of P9B were used as parents in multiple backcross generations to produce P9SA, a CMS line with stable sterility, to explore the molecular mechanisms of the association between GMS and CMS. The anthers of the maintainer (P9B), GMS (P9BS), and CMS (P9SA) lines were compared through phenotypic, cell morphological, physiological, biochemical observations, and transcriptome analysis. Premature degradation of the tapetum was observed at the mononuclear stage in P9BS and P9SA, which also had lower activity of reactive oxygen species (ROS) scavenging enzymes compared with P9B. Many coexpressed differentially expressed genes were related to ROS balance, including ATP synthase, electron chain transfer, and ROS scavenging processes were upregulated in P9B. CMS plants had a higher ROS accumulation than GMS plants. The MDA content in P9SA was 3.2 times that of P9BS, and therefore, a higher degree of abortion occurred in P9SA, which may indicate that the conversion between CMS and GMS is related to intracellular ROS accumulation. Our study adds new insights into the natural transformation of GMS and CMS in plants in general and kenaf in particular.
雄性不育（MS）在植物杂种生产中起着关键作用。研究人员关注了麻雄性不育（GMS）和细胞质雄性不育（CMS）之间的关联。在这项研究中，P9BS（麻系 P9B 的天然 GMS 突变体）和 P9B 的雄性植物被用作亲本，经过多次回交世代，产生了具有稳定不育性的 CMS 系 P9SA，以探索 GMS 和 CMS 之间关联的分子机制。通过表型、细胞形态、生理、生化观察和转录组分析比较了保持系（P9B）、GMS（P9BS）和 CMS（P9SA）系的花药。在 P9BS 和 P9SA 中，绒毡层在单核期观察到过早降解，与 P9B 相比，其活性氧（ROS）清除酶活性也较低。许多共表达的差异表达基因与 ROS 平衡有关，包括 ATP 合酶、电子链传递和 ROS 清除过程在 P9B 中上调。CMS 植物比 GMS 植物积累更高的 ROS。P9SA 中的 MDA 含量是 P9BS 的 3.2 倍，因此 P9SA 发生了更高程度的流产，这可能表明 CMS 和 GMS 之间的转换与细胞内 ROS 积累有关。我们的研究为植物特别是麻中 GMS 和 CMS 的自然转化提供了新的见解。
