Jiang Jingyi, Chen Chen, Cheng Shaoze, Yuan Xia, Jin Jing, Zhang Chen, Sun Xiaolin, Song Jiuzhou, Zuo Qisheng, Zhang Yani, Chen Guohong, Li Bichun
Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China.
Animal & Avian Sciences, University of Maryland, College Park, MD 20741, USA.
Animals (Basel). 2021 Jan 24;11(2):292. doi: 10.3390/ani11020292.
Although lncRNAs have been identified as playing critical roles in the development of germ cells, their potential involvement in the development of PGCs in chickens remains poorly understood. Differentially expressed lncRNAs (DELs) from previous RNA-seq of embryonic stem cells (ESCs), PGCs, and spermatogonial stem cells (SSCs) were analyzed by K-means clustering, from which a key candidate, lncRNA (lncRNA PGC regulator, ) was obtained. We confirmed that plays a positive role in the development of PGCs by increasing the expression of the PGC marker gene ( and ), while downregulating the pluripotency-associated gene () in vitro and in vivo. The activation and expression of are regulated by histone acetylation, and transcription factor TCF7L2. Mechanistically, a rescue assay was performed to further confirm that contributed to the development of PGCs by regulating the gga-miR-6577-5p/ signaling pathway. Adsorption of gga-miR-6577-5p activated the WNT signaling cascade by relieving the gga-miR-6577-5p-dependent inhibition of expression. Taken together, our study discovered the growth-expedited role of in PGCs development, showing the potential /miR-6577-5p/ pathway. The results and findings provide a novel insight into the development of PGCs.
尽管长链非编码RNA（lncRNAs）已被确定在生殖细胞发育中发挥关键作用，但其在鸡原始生殖细胞（PGCs）发育中的潜在作用仍知之甚少。通过K均值聚类分析了先前胚胎干细胞（ESCs）、PGCs和精原干细胞（SSCs）RNA测序中差异表达的lncRNAs（DELs），从中获得了一个关键候选lncRNA（lncRNA PGC调节因子）。我们证实，该lncRNA在体外和体内通过增加PGC标记基因（和）的表达，同时下调多能性相关基因（），在PGCs发育中发挥积极作用。该lncRNA的激活和表达受组蛋白乙酰化和转录因子TCF7L2调控。从机制上讲，进行了一项拯救试验，以进一步证实该lncRNA通过调节gga-miR-6577-5p/信号通路促进PGCs的发育。吸附gga-miR-6577-5p可通过解除gga-miR-6577-5p对该lncRNA表达的依赖性抑制来激活WNT信号级联反应。综上所述，我们的研究发现了该lncRNA在PGCs发育中的促生长作用，揭示了潜在的/miR-6577-5p/信号通路。这些结果和发现为PGCs的发育提供了新的见解。
