Shapiro D J, Sharp P A, Wahli W W, Keller M J
Department of Biochemistry, University of Illinois, Urbana 61801.
DNA. 1988 Jan-Feb;7(1):47-55. doi: 10.1089/dna.1988.7.47.
A HeLa cell nuclear transcription extract that is approximately 20 times more efficient than standard HeLa cell transcription extracts was developed. Transcription of the strong adenovirus II major late promoter by this extract results in the synthesis of 1.5-4 molecules of product RNA per molecule of template, indicating that the extract is capable of multiple rounds of initiation. Standard HeLa cell nuclear extracts transcribe closed circular and linear adenovirus major late promoter templates with equal efficiency. In contrast, the new extract exhibits an increase of approximately twofold on transcription of a closed circular, as opposed to a linear, major late promoter template.
开发出一种比标准的HeLa细胞核转录提取物效率高约20倍的HeLa细胞核转录提取物。该提取物对腺病毒II型主要晚期强启动子的转录导致每分子模板合成1.5 - 4分子产物RNA，这表明该提取物能够进行多轮起始。标准的HeLa细胞核提取物以相同效率转录闭环和线性腺病毒主要晚期启动子模板。相比之下，新提取物对闭环而非线性主要晚期启动子模板的转录效率提高了约两倍。
