Sándor Attila D, Péter Áron, Corduneanu Alexandra, Barti Levente, Csősz István, Kalmár Zsuzsa, Hornok Sándor, Kontschán Jenő, Mihalca Andrei D
Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, RO-400036 Cluj Napoca, Romania.
Department of Parasitology and Zoology, University of Veterinary Medicine, H-1078 Budapest, Hungary.
Microorganisms. 2021 Jan 22;9(2):230. doi: 10.3390/microorganisms9020230.
Malaria is responsible for major diseases of humans, while associated haemosporidians are important factors in regulating wildlife populations. , a haemosporidian parasite of bats, is phylogenetically close to human-pathogenic species, and their study may provide further clues for understanding the evolutionary relationships between vertebrates and malarial parasites. Our aim was to investigate the distribution of spp. in Eastern Europe and test the importance of host ecology and roost site on haemosporidian parasite infection of bats. We sampled bats and their ectoparasites at eight locations in Romania and Bulgaria. DNA was extracted from blood samples and ectoparasites and tested individually for the presence of DNA of spp. using a nested PCR targeting a 705 bp fragment of B. Two species of were identified: in and associated ectoparasites and in rhinolophid and vespertilionid bats (6 species) and their ticks and nycteribiid flies. Only cave-dwelling bat species (and their ectoparasites) showed infections, and we found a strong correlation between infections with parasites and Nycteribiidae prevalence. We report the high genetic diversity of spp. in Eastern Europe, suggesting that the simultaneous presence of varied host and vector assemblages enhances bat haemosporidian parasite diversity.
疟疾是导致人类重大疾病的原因，而相关的血孢子虫是调节野生动物种群数量的重要因素。蝙蝠血孢子虫是蝙蝠的一种血孢子虫寄生虫，在系统发育上与人类致病物种相近，对它们的研究可能为理解脊椎动物与疟原虫之间的进化关系提供进一步线索。我们的目的是调查东欧地区蝙蝠血孢子虫的分布情况，并测试宿主生态和栖息地对蝙蝠血孢子虫感染的重要性。我们在罗马尼亚和保加利亚的八个地点对蝙蝠及其体外寄生虫进行了采样。从血液样本和体外寄生虫中提取DNA，并使用针对巴氏血孢子虫705 bp片段的巢式PCR分别检测蝙蝠血孢子虫DNA的存在情况。鉴定出两种蝙蝠血孢子虫：一种存在于菊头蝠及其相关体外寄生虫中，另一种存在于菊头蝠科和蝙蝠科蝙蝠（6种）及其蜱虫和蝠蝇中。只有栖息在洞穴中的蝙蝠物种（及其体外寄生虫）出现感染情况，并且我们发现感染蝙蝠血孢子虫与蝠蝇科的流行率之间存在很强的相关性。我们报告了东欧地区蝙蝠血孢子虫的高遗传多样性，这表明不同宿主和媒介组合的同时存在增加了蝙蝠血孢子虫寄生虫的多样性。
