Labrecque C, Tremblay J P, Fahim M A
Department of Anatomy, Laval University, Quebec, Canada.
Exp Neurol. 1988 Apr;100(1):51-64. doi: 10.1016/0014-4886(88)90200-2.
The structure of the end-plate regions of normal and dystrophic 3-month-old mice were studied by scanning electron microscopy after the presynaptic terminals were removed by hydrochloric acid treatment. Quantitative analysis revealed that the end-plate area correlated positively with the muscle fiber diameter in both the normal and dystrophic animals. However, the motor end-plate area was significantly smaller in the dystrophic mice. The total length of the primary cleft of an end-plate correlated positively with the end-plate area and with the muscle fiber diameter in both normal and dystrophic mice. However, the total length of the primary cleft of an end-plate was significantly shorter in dystrophic mice, especially in large-diameter muscle fibers. Finally, the end-plate of dystrophic mice was characterized by shorter primary clefts with less branching points. These changes of several morphometric characteristics of the postsynaptic membrane suggest that the functional denervation of the mouse dystrophic neuromuscular junction has a postsynaptic origin.
在通过盐酸处理去除突触前终末后，利用扫描电子显微镜对3月龄正常和营养不良小鼠的终板区域结构进行了研究。定量分析显示，正常和营养不良动物的终板面积均与肌纤维直径呈正相关。然而，营养不良小鼠的运动终板面积明显较小。正常和营养不良小鼠终板初级裂隙的总长度均与终板面积和肌纤维直径呈正相关。然而，营养不良小鼠终板初级裂隙的总长度明显较短，尤其是在大直径肌纤维中。最后，营养不良小鼠的终板特点是初级裂隙较短且分支点较少。突触后膜这些形态测量特征的变化表明，小鼠营养不良性神经肌肉接头的功能性失神经起源于突触后。
