Hellung-Larsen P
Department of Biochemistry B, University of Copenhagen, Denmark.
Experientia. 1988 Jan 15;44(1):58-60. doi: 10.1007/BF01960245.
In cultures with efficient aeration a maximum cell concentration (MCC) of 6 X 10(5) cells/ml (defined medium) and 5.5 X 10(6) cells/ml (broth) can be reached. By culturing within Millicells with excess supply of medium and efficient removal of waste products a physical limit for MCC of about 13 X 10(6) cells/ml is reached.