Laboratory of Microbial Biotechnologies, Agrosciences and Environment (BioMAgE), Faculty of Sciences Semlalia, Cadi Ayyad University, PO Box 2390, Marrakesh, Morocco.
Laboratory of Microbiology and Virology, Faculty of Medicine and Pharmacy, Cadi Ayyad University, PO Box 7010, Marrakesh, Morocco.
Environ Sci Pollut Res Int. 2021 Jun;28(21):26840-26848. doi: 10.1007/s11356-021-12445-4. Epub 2021 Jan 26.
The efficiency of the treatment of hospital wastewater by actinobacteria was investigated using two chemometric data analysis methods. Six strains of multi-resistant bacteria isolated from Marrakesh hospital wastewater and four strains of antagonistic actinobacteria isolated from Moroccan marine environment were characterized by fatty acids released as methyl esters by thermochemolysis-GC/MS. The hierarchical cluster analysis (HCA) and the principal component analysis (PCA) were used to correlate fatty acids (FA) distributions within strains. HCA allowed to discriminate between bacteria and actinobacteria. A lower Euclidean distance is noted for bacteria. With PCA, linear and branched-chained FAs correlated with bacteria whereas mono unsaturated FAs correlated more specifically with Gram (-) bacteria. Terminally branched-chained FAs correlated most likely with actinobacteria. A co-culture of actinobacteria and bacteria monitored during 15 days demonstrated the efficiency of the biological treatment for 2 of the 4 studied actinobacteria. The effect is more important on Gram-negative bacteria. Antagonistic actinobacteria seem to be poorly efficient against Gram-positive bacteria.
采用两种化学计量数据分析方法研究了放线菌对医院废水的处理效率。从马拉喀什医院废水中分离出的 6 株多耐药菌和从摩洛哥海洋环境中分离出的 4 株拮抗放线菌，通过热化学解-气相色谱/质谱法释放出的脂肪酸甲酯进行了特征描述。采用层次聚类分析（HCA）和主成分分析（PCA）对菌株内脂肪酸（FA）分布进行相关性分析。HCA 可区分细菌和放线菌。细菌的欧几里得距离较低。通过 PCA，线性和支链脂肪酸与细菌相关，而单不饱和脂肪酸与革兰氏阴性菌更相关。末端支链脂肪酸与放线菌最相关。放线菌和细菌的共培养在 15 天内进行监测，结果表明，在研究的 4 种放线菌中，有 2 种具有生物处理的效率。这种效果对革兰氏阴性菌更为重要。拮抗放线菌对革兰氏阳性菌的效率似乎较差。
