冷冻精子实现最佳存活及体外受精能力所需的甘油浓度取决于冷冻保存培养基。

Concentration of glycerol required for optimal survival and in vitro fertilizing capacity of frozen sperm is dependent on cryopreservation medium.

作者信息

Hammitt D G, Walker D L, Williamson R A

机构信息

Department of Obstetrics and Gynecology, University of Iowa Hospitals and Clinics, Iowa City 52242.

出版信息

Fertil Steril. 1988 Apr;49(4):680-7. doi: 10.1016/s0015-0282(16)59840-9.

DOI:10.1016/s0015-0282(16)59840-9

PMID:3350164

Abstract

Sperm survival and in vitro fertilizing capacity were examined following cryopreservation with three concentrations of glycerol (0, 2, and 7.5%) and four cryopreservation media. Post-thaw motility and motility index increased with increasing concentrations of glycerol. Post-thaw velocity, linearity, percentage of zona-free hamster oocytes penetrated, and penetrations per oocyte were greater following freezing with 2 or 7.5% glycerol than with 0% glycerol. A significant interaction between media and glycerol was observed for motility, velocity, linearity, motility index, and percentage of oocytes penetrated. These results suggest that the concentration of glycerol required for optimal survival and in vitro fertilizing capacity of human sperm following cryopreservation is dependent on the type of medium used for freezing.

摘要

使用三种甘油浓度（0%、2%和7.5%）以及四种冷冻保存培养基进行冷冻保存后，对精子的存活率和体外受精能力进行了检测。解冻后的活力和活力指数随甘油浓度的增加而升高。与0%甘油冷冻相比，用2%或7.5%甘油冷冻后，解冻后的速度、直线性、去透明带仓鼠卵母细胞穿透率和每个卵母细胞的穿透数更高。在活力、速度、直线性、活力指数和卵母细胞穿透率方面，观察到培养基和甘油之间存在显著的相互作用。这些结果表明，冷冻保存后人精子最佳存活和体外受精能力所需的甘油浓度取决于用于冷冻的培养基类型。