冷冻保存对人精子穿透牛宫颈黏液能力的影响。
Effects of cryopreservation on penetration ability of human spermatozoa into bovine cervical mucus.
作者信息
Esfandiari N, Rouzrokh A, Saremi A T
机构信息
Department of Reproductive Immunology, Shaheed Beheshti University of Medical Sciences, Tehran, Iran.
出版信息
Arch Androl. 2000 Nov-Dec;45(3):233-8. doi: 10.1080/01485010050194011.
Cryopreserved sperm exhibit lower fertilizing capacity in comparison to fresh sperm, partly due to effects of glycerol as the common cryoprotectant medium. Since standard semen analysis is not a good predictive method to assess sperm fertilizing capacity, functional tests like cervical mucus penetration may provide more useful information. A total of 24 semen samples were examined before and after cryopreservation for sperm parameters as well as number and motility of penetrated sperm into bovine cervical mucus (BCM) as an alternative for human cervical mucus. Freezing and thawing procedures have negative effects on sperm penetration into cervical mucus. No significant relation was noticed between sperm motility percentage or its penetration into BCM before and after cryopreservation, which denotes the variability in resistance of sperm to damaging effects of freezing.
与新鲜精子相比，冷冻保存的精子受精能力较低，部分原因是甘油作为常用冷冻保护剂介质所产生的影响。由于标准精液分析并非评估精子受精能力的良好预测方法，像宫颈黏液穿透试验这样的功能测试可能会提供更有用的信息。总共对24份精液样本在冷冻保存前后进行了检查，以检测精子参数以及穿透牛宫颈黏液（作为人宫颈黏液的替代物）的精子数量和活力。冷冻和解冻过程对精子穿透宫颈黏液有负面影响。在冷冻保存前后，未观察到精子活力百分比或其穿透牛宫颈黏液的情况之间存在显著关系，这表明精子对冷冻损伤作用的抵抗力存在变异性。
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