MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, China.
Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, China.
J Thromb Haemost. 2021 Aug;19(8):2029-2043. doi: 10.1111/jth.15252. Epub 2021 Jun 16.
Chemotherapy-induced thrombocytopenia (CIT) can increase the risk of bleeding, which may delay or prevent the administration of anticancer treatment schedules. Photobiomodulation therapy (PBMT), a non-invasive physical treatment, has been proposed to improve thrombocytopenia; however, its underlying regulatory mechanism is not fully understood.
To further investigate the mechanism of thrombopoietin (TPO) in megakaryocytopoiesis and thrombopoiesis.
Multiple approaches such as western blotting, cell transfection, flow cytometry, and animal studies were utilized to explore the effect and mechanism of PBMT on thrombopoiesis.
PBMT prevented a severe drop in platelet count by increasing platelet production, and then ameliorated CIT. Mechanistically, PBMT significantly upregulated hepatic TPO expression in a thrombocytopenic mouse model, which promoted megakaryocytopoiesis and thrombopoiesis. The levels of TPO mRNA and protein increased by PBMT via the Src/ERK/STAT3 signaling pathway in hepatic cells. Furthermore, the generation of the reactive oxygen species was responsible for PBMT-induced activation of Src and its downstream target effects.
Our research suggests that PBMT is a promising therapeutic strategy for the treatment of CIT.
化疗诱导的血小板减少症（CIT）会增加出血风险，这可能会延迟或阻碍抗癌治疗方案的实施。光生物调节疗法（PBMT）作为一种非侵入性的物理治疗方法，已被提出用于改善血小板减少症；然而，其潜在的调节机制尚不完全清楚。
深入研究血小板生成素（TPO）在巨核细胞生成和血小板生成中的作用。
采用 Western blot、细胞转染、流式细胞术和动物研究等多种方法，探讨 PBMT 对血小板生成的作用及其机制。
PBMT 通过增加血小板生成来预防血小板计数的严重下降，从而改善 CIT。从机制上讲，在血小板减少症小鼠模型中，PBMT 显著上调了肝脏中的 TPO 表达，从而促进了巨核细胞生成和血小板生成。在肝细胞中，PBMT 通过Src/ERK/STAT3 信号通路增加了 TPO mRNA 和蛋白的水平。此外，活性氧的产生负责 PBMT 诱导的Src 激活及其下游靶标效应。
我们的研究表明，PBMT 是治疗 CIT 的一种很有前途的治疗策略。
